Abstract
This paper describes rapid propagation of Sesbania drummondii using nodal explants isolated from seedlings and young plants. The nodal segments proliferated into multiple shoots on Murashige and Skoog's (MS) medium supplemented with 22.2 μM benzyladenine. MS medium containing 2.2 and 4.5 μM thidiazuron induced 5 – 6 shoots per stem node from 3-month-old plants. Nodal explants when cultured on MS medium containing combinations of benzyladenine (8.8 and 11.1 μM) and indole-3-butyric acid (0.24 – 2.46 μM) or indole-3-acetic acid (0.28 – 2.85 μM) gave lesser number of shoots. Callus induced on cotyledonary explants when subcultured on 2.2 μM thidiazuron containing medium resulted in its mass proliferation having numerous embryoid-like structures. Indole-3-butyric acid (0.24 – 2.46 μM) was found suitable for root induction. In vitro regenerated plants were acclimatized in greenhouse conditions.
Similar content being viewed by others
References
Agrawal, V., Prakash, S., Gupta, S.C.: Effective protocol for in vitro shoot production through nodal explants of Simmondsia chinesis.-Biol. Plant. 45: 449-453, 2002.
Bansal, Y.K., Pandey, P.: Micropropagation of Sesbania aculeata (Pers.) by adventitious organogenesis.-Plant Cell Tissue Organ Cult. 32: 352-355, 1993.
Detrez, C., Ndiaye, S., Dreyfus, B.: In vitro regeneration of the tropical multipurpose leguminous tree Sesbania grandiflora from cotyledon explants.-Plant Cell Rep. 14: 87-93, 1994.
Harris, P.J.C., Puddephat, I.J.: In vitro propagation of Sesbania species.-Nitrogen Fixing Tree Res. Rep. 7: 129-131, 1989.
Kapoor, S., Gupta, S.C.: Rapid in vitro differentiation of Sesbania bispinosa plants -a leguminous shrub.-Plant Cell Tissue Organ Cult. 7: 263-268, 1986.
Khattar, S., Mohan Ram, H.Y.: Organogenesis in the cultured tissues of Sesbania sesban, a leguminous shrub.-Indian J. exp. Biol. 19: 216-219, 1982.
Khattar, S., Mohan Ram, H.Y.: Organogenesis and plantlet formation in vitro in Sesbania grandiflora (L.) Pers.-Indian J. exp. Biol. 21: 251-253, 1983.
Malik, K.A., Saxena, P.K.: TDZ induces high-frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum), and lentil (Lens culinaris).-Aust. J. Plant Physiol. 19: 731-740, 1992.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962.
Murthy, B.N.S., Murch, S.J., Saxena, P.K.: Thidiazuron: a potent regulator of in vitro plant morphogenesis.-In vitro cell. dev. Biol. Plant 34: 267-275, 1998.
Pandey, P., Bansal, Y.K.: Plant regeneration from leaf and hypocotyl explants from Glycine wightii (W. and A.) Verde. var. longicauda.-Jap. J. Breed. 42: 1-5, 1992.
Powell, R.G., Smith, C.R.: An investigation of the anti-tumor activity of Sesbania drummondii.-J. natur. Prod. 44: 86-90, 1981.
Sahi, S.V., Bryant, N.L., Sharma, N.C., Singh, S.R.: Characterization of a lead hyperaccumulator shrub, Sesbania drummondii.-Environ. Sci. Technol. 36: 4676-4680, 2002.
Saritha, K.V., Prakash, E., Ramamurty, N., Naidu, C.V.: Micropropagation of Spilanthes acmella Murr.-Biol. Plant. 45: 581-584, 2002.
Shahana, S., Gupta, S.: Somatic embryogenesis in Sesbania sesban var. bicolor: a multipurpose fabaceous woody species.-Plant Cell Tissue Organ Cult. 69: 289-292; 2002.
Shanker, S., Mohan Ram, H.Y.: Plantlet regeneration from tissue culture of Sesbania grandiflora.-Curr. Sci. 59: 39-43, 1990.
Sinha, R.K., Mallick, R.: Plantlets from somatic callus tissue of the woody legume Sesbania bispinosa (Jacq.) W.F. Wight.-Plant Cell Rep. 10: 247-250, 1991.
Vlachova, M., Merz, B.A., Schell, J., De Brujin, F.J.: The tropical legume Sesbania rostrata: tissue culture, plant regeneration and infection with Agrobacterium tumefaciens and rhizogenes strains.-Plant Sci. 50: 213-223, 1987.
Xu, Z.H., Yang, L.J., Wai, A., Gao, M.: Plant regeneration in tissue culture of four legumes.-Acta biol. exp. sin. 17: 483-489, 1984.
Zhao, Y.-X., Yao, D.-Y., Harris, P.J.C.: Plant regeneration from callus and explants of Sesbania spp.-Plant Cell Tissue Organ Cult. 34: 253-260, 1993.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cheepala, S., Sharma, N. & Sahi, S. Rapid in vitro Regeneration of Sesbania drummondii . Biologia Plantarum 48, 13–18 (2004). https://doi.org/10.1023/B:BIOP.0000024269.72171.42
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000024269.72171.42