Skip to main content

Growth and Protein Pattern in Cowpea Seedlings Subjected to Salinity

Abstract

Cowpea (Vigna unguiculata) seeds were put to germinate on filter paper under control (distilled water) and salt stress (100 mM MaCl) conditions. Seeds and seedlings were classified in eight developmental stages (DS), according to their morphological traits. Under control conditions, 7 d after planting, 100 % of the seedlings reached DS VIII (seedlings with radicles measuring more than 5 cm, cotyledons leaving the filter paper, hypocotyls straight and cotyledonary leaves fully open) and under NaCl stress conditions, 11 d after planting only 68 % of the seedlings were at DS VIII. The length of the main root and of shoot has decreased 23 and 44 %, respectively. The two-dimensional electrophoretic patterns of the albumins isolated from stems and leaves were determined in seedlings at DS VIII. In stems 19 proteins (14.6 to 76.3 kDa) had their relative concentration increased by salinity, 8 (31.2 to 65.0 kDa) had their relative concentration decreased by salinity and 9 (16.3 to 39.8 kDa) were apparently synthesised de novo. In leaves, under salt conditions 9 proteins (18.2 to 33.2 kDa) increased in concentration, one (17.1 kDa) decreased in concentration and one (21.2 kDa) was apparently synthesised de novo.

This is a preview of subscription content, access via your institution.

References

  1. Amzallag, G.N.: Tolerance to salinity in plants: new concepts for old problems.-In: Jaiwal, P.K., Singh, R.P., Gulati, A. (ed.): Strategies for Improving Salt Tolerance in Higher Plants. Pp. 1–24. Oxford & IBH Publishing Co., New Delhi 1997.

    Google Scholar 

  2. Amzallag, G.N., Lerner, R.: Physiological adaptation of plants to environmental stress.-In: Pessarakli, M. (ed.): Handbook of Plant and Crop Physiology. Pp. 557–576. Marcel Dekker, New York 1994.

    Google Scholar 

  3. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.

    PubMed  CAS  Article  Google Scholar 

  4. Cainnes, A.M., Shennam, C. Interactive effects of Ca2+ and NaCl salinity on the growth of two tomato genotypes differing in Ca2+ use efficiency.-Plant Physiol. Biochem. 37: 569–576, 1999.

    Google Scholar 

  5. Dash, M., Panda, S.K.: Salt stress induced changes in growth and enzyme activities in germinating Phaseolus mungo seeds.-Biol. Plant. 44: 587–589, 2001.

    CAS  Article  Google Scholar 

  6. El-Shintinawy, F., El-Shourbagy, M.N.: Alleviation of changes in protein metabolism in NaCl-stressed wheat seedlings by thiamine.-Biol. Plant. 44: 541–545, 2001.

    CAS  Article  Google Scholar 

  7. Franco, O.L., Enèas-Filho, J., Prisco, J.T., Gomes-Filho, E.: Effects of CaCl2 on growth and osmoregulator accumulation in NaCl stressed cowpea seedlings.-Rev. bras. Fisiol. veg. 11: 145–151, 1999.

    CAS  Google Scholar 

  8. Gomes-Filho, E., Enèas-Filho, J., Prisco, J.T.: Effects of osmotic stress on growth and ribonuclease activity in Vigna unguiculata (L.) Walp. seedlings differing in stress tolerance.-Rev. bras. Fisiol. veg. 8: 51–57, 1996.

    CAS  Google Scholar 

  9. Gomes-Filho, E., Prisco, J.T., Campos, F.A.P., Enèas-Filho, J.: Effects of salinity in vivo and in vitro on ribonuclease activity of Vigna unguiculata cotyledons during germination.-Physiol. Plant. 59: 183–188, 1983.

    CAS  Article  Google Scholar 

  10. Greenway, H., Munns, R.: Mechanisms of salt tolerance in nonhalophytes.-Annu. Rev. Plant Physiol. 31: 149–190, 1980.

    CAS  Article  Google Scholar 

  11. Hurkman, W.J., Tanaka, C.K.: The effects of salt on the pattern of protein syntesis in barley roots.-Plant Physiol. 83: 517–524, 1987.

    PubMed  CAS  Article  Google Scholar 

  12. Iuchi, S., Yamaguchi-Shinozaki, K., Urao, T., Terao, T., Shinozaki, K.: Novel drought-inducible genes in the highly drought-tolerant cowpea: cloning of cDNAs and analysis of expression of the corresponding genes.-Plant Cell Physiol. 37: 1073–1082, 1996.

    PubMed  CAS  Google Scholar 

  13. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680–685, 1970.

    PubMed  CAS  Article  Google Scholar 

  14. Maas, E.V., Hoffmann, G.J.: Crop salt tolerance-current assesment.-J. Irrig. Drain. Div. 103: 115–134, 1977.

    Google Scholar 

  15. O'Farrell, P.H.: High resolution two-dimensional electrophoresis.-J. biol. Chem. 250: 4007–4021, 1975.

    PubMed  Google Scholar 

  16. Poljakoff-Mayber, A.: Morphological and anatomical changes in plants as a response to salinity stress.-In: Poljakoff-Mayber, A., Gale, J. (ed.): Plants in Saline Environments. Pp. 97–117. Springer-Verlag, New York 1975.

    Google Scholar 

  17. Prisco, J.T., Vieira, G.H.F. Effects of NaCl salinity on nitrogenous compounds and proteases during germination of Vigna sinensis seeds.-Physiol. Plant. 36: 317–320, 1976.

    CAS  Article  Google Scholar 

  18. Ramagopal, S.: Differential mRNA transcription during salinity stress in barley.-Proc. nat. Acad. Sci. USA 84: 94–98, 1987.

    PubMed  CAS  Article  Google Scholar 

  19. Ramani, S., Apte, S.K.: Transient expression of multiple genes in salinity-stressed young seedlings of rice (Oryza sativa L.) cv. Bura Rata.-Biochem. biophys. Res. Commun. 233: 663–667, 1997.

    PubMed  CAS  Article  Google Scholar 

  20. Ricchardi, F., Gazeu, P., Vienne, D., Zivy, M.: Protein changes in response to progressive water deficit in maize.-Plant Physiol. 117: 1254–1263, 1998.

    Google Scholar 

  21. Sabehat, A., Weiss, D., Lurie, S.: Heat-shock proteins and cross-tolerance in plants.-Physiol. Plant. 103: 437–441, 1998.

    CAS  Article  Google Scholar 

  22. Schevchenko, A., Wilm, M., Vorm., O., Mann, M.: Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.-Anal. Chem. 68: 850–858, 1996.

    Article  Google Scholar 

  23. Sohan, D., Jasoni, R., Zajicek, J.: Plant-water relations of NaCl and calcium-treated sunflower plants.-Environ. exp. Bot. 42: 105–111, 1999.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sousa, M., Campos, F., Prisco, J. et al. Growth and Protein Pattern in Cowpea Seedlings Subjected to Salinity. Biologia Plantarum 47, 341–346 (2003). https://doi.org/10.1023/B:BIOP.0000023875.63226.67

Download citation

  • 2-D electrophoresis
  • NaCl-stress
  • Vigna unguiculata