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Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase

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Abstract

Rhodococcus pyridinovorans MW3 was isolated from an arable land of manioc from the Congo for its ability to transform acrylonitrile to acrylamide. This strain contains a cobalt nitrile hydratase (NHase) showing high sequence homology with NHases so far described. The specific NHase activity was 97 U mg−1 dry wt. NHase production by R. pyridinovorans MW3 was urea and Co-dependent. The NHase was active for acrylamide up to 60% (w/v) indicating its potential for acrylamide production.

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Authors and Affiliations

  1. Laboratoire d'Ecologie Moléculaire, IBEAS, EA 3525, Université de Pau et des Pays de l'Adour, avenue de l'Université, UFR Sciences et Techniques, BP 1155 F-64013, Pau cedex, France

    Sylvain Precigou, Marco Wieser, Philippe Goulas & Robert Duran

  2. Aventis Pasteur, 1541 avenue Marcel Merieux, 69280, Marcy l'Etoile, France

    Patrick Pommares & Robert Duran

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  1. Sylvain Precigou
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  2. Marco Wieser
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  3. Patrick Pommares
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  4. Philippe Goulas
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  5. Robert Duran
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Precigou, S., Wieser, M., Pommares, P. et al. Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase. Biotechnology Letters 26, 1379–1384 (2004). https://doi.org/10.1023/B:BILE.0000045636.47527.99

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  • DOI: https://doi.org/10.1023/B:BILE.0000045636.47527.99

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