Abstract
A simple one-step purification method, using expanded bed, ion-exchange chromatography, for the fractionation of nisin Z produced by Lactococcus lactis subsp. lactis A164 was developed. The highest dynamic binding capacity (0.92) of the adsorbent was obtained at a superficial velocity of 367 cm h−1, resulting in approx. 2.7-fold bed expansion. The range of pH for the maximum adsorption was 3–4. The isocratic elution with 0.15 m NaCl led to approx. >90% recovery. Single-step purification of nisin Z from unclarified A164 culture broth resulted in 31-fold purification with a 90% yield.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhunia AK, Johnson MC, Ray B (1987) Direct detection of an anti-microbial peptide of Pediococcus acidilactici in sodium dodesyl sulfate polyacrylamide gel electrophoresis. J. Ind. Microbiol. 2: 319-322.
Carolissen-Mackay V, Arendse G, Hastings JW (1997) Purification of bacteriocins of lactic acid bacteria: problems and pointers. Int. J. Food Microbiol. 34: 1-16.
Chang YK, Chase HA (1996a) Ion exchange purification of G6PDH from unclarified yeast cell homogenates using expanded bed adsorption. Biotechnol. Bioeng. 49: 204-216.
Chang YK, Chase HA (1996b) Development of operating conditions for protein purification using expanded bed techniques: the effect of the degree of bed expansion on adsorption performance. Biotechnol. Bioeng. 49: 512-526.
Cheigh CI, Choi HJ, Park H, Kim SB, Kook MC, Kim TS, Hwang JK, Pyun YR (2002) Influence of growth conditions on the production of a nisin-like bacteriocin by lactococcus lactis subsp. lactis A164 isolated from kimchi. J. Biotechnol. 95: 225-235.
Choi HJ, Cheigh CI, Kim SB, Pyun YR (2000) Production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from Kimchi. J. Appl. Microbiol. 88: 1-11.
De Vuyst L, Vandamme EJ (1994) Bacteriocins of Lactic Acid Bacteria; Microbiology, Genetics and Applications. London: Blackie Academic & Professional.
Hu HB, Yao SJ, Mei LH, Zhu ZQ, Hur BK (2000) Partial purification of nattokinase from Bacillus subtilis by expanded bed adsorption. Biotechnol. Lett. 22: 1383-1387.
Jack RW, Tagg JR, Ray B (1995) Bacteriocins of Gram-positive bacteria. Microbiol. Rev. 59: 171-200.
Mullick A, Flickinger MC (1999) Expanded bed adsorption of human serum albumin from very dense Saccharomyces cerevesiae suspensions on fluoride-modified zirconia. Biotechnol. Bioeng. 65: 282-290.
Parente E, Ricciardi A (1999) Production, recovery and purification of bacteriocins from lactic acid bacteria. Appl. Microbiol. Biotechnol. 52: 628-638.
Ray B, Daeschel MA (1992) Food Biopreservatives of Microbial Origin. Boca Raton, FL: CRC Press.
Yang R, Johnson MC, Ray B (1992) Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl. Environ. Microbiol. 58: 3355-3359.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cheigh, CI., Kook, MC., Kim, SB. et al. Simple one-step purification of nisin Z from unclarified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography. Biotechnology Letters 26, 1341–1345 (2004). https://doi.org/10.1023/B:BILE.0000045630.29494.45
Issue Date:
DOI: https://doi.org/10.1023/B:BILE.0000045630.29494.45