Abstract
The objective of this research was to compare different methods for the separation and purification of hyaluronic acid from Streptococcus zooepidemicus ATCC39920 for their ease of use and reliability. The most appropriate conditions of pretreatment stage were heated to 70 °C for 1 h and kept at room temperature for 5 h. The fermented broth was diluted with the equal volume deionized water. Ten milligrams chitosan was slowly added to 1 L of solution with stirring for 30 min, followed by the addition 20 g diatomaceous earth-type filter aid. The mixture was stirring for 1 h. Then, the mixture was filtrated with diatomite, microfiltration (MF) membranes (5 μm in pore diameter), and ultrafiltration (UF) membranes (MWCO 50KD) to achieve the separation stage.The retentate was washed by the equal volume deionized water and when the volume concentration ratio (VCR) reached 4, the UF stage ended. Finally, the purification procedure adopted Sevag process to remove protein. The overall yield of HA could reach 62.53 % and the removal rate of protein could reach 95.22 %.
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Acknowledgments
This work was supported by 7th Singapore–China JRP (no. 2011DFA31280), the Nation Science Foundation of China (no. 31101275), and a research grant (no. 20100207) from Tianjin University of Science and Technology, China.
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Wang, Y., Zhang, J., Liu, H. (2014). Separation and Purification of Hyaluronic Acid from Fermentation Broth. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_162
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DOI: https://doi.org/10.1007/978-3-642-37925-3_162
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