Abstract
The maximum product yield of endo-glucanase (650 IU g−1 substrate) from Cellulomonas biazotea mutant 51 Smr was 1.5- to 2.5-fold more than was produced by the wild type cells and was twice that reported by previous researchers. Mutation substantially improved the enthalpy (ΔH *) and entropy of activation (ΔS *) for product formation, turnover number, specificity constant activation energy, free energies for transition state formation and substrate binding for CMC hydrolysis respectively.
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Rajoka, M., Ashraf, Y. & Khalid, A. Kinetic of improved production and carboxymethyl cellulose hydrolysis by an endo-glucanase from a derepressed mutant of Cellulomonas biazotea . Biotechnology Letters 26, 1329–1333 (2004). https://doi.org/10.1023/B:BILE.0000045628.32242.99
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DOI: https://doi.org/10.1023/B:BILE.0000045628.32242.99