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Comparative Analysis of the Properties of Recombinant Endo-1,4-β-Glucanase II and Its Chimeric Form with a Cellular Binding Module

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Abstract

A chimeric form of Penicillium verruculosum endo-1,4-β-glucanase II (EGII), the C-terminus of which contains a cellulose-binding module (CBM) of P. verruculosum cellobiohydrolase I, is produced by the genetic engineering method. In the native form, the eglI gene does not have a region encoding CBM. The resulting chimeric enzyme is isolated in a homogeneous form and its properties are studied. The addition of CBM to EGII led to a significant increase in the activity of the chimeric enzyme relative to microcrystalline cellulose (MCC) and the appearance of its adsorption capacity in relation to cellulose. However, the addition of CBM to EGII led to a decrease in activity towards soluble polysaccharide substrates (carboxymethylcellulose and β-glucan). The chimeric form of the enzyme in the composition of the cellulase complex allowed us to hydrolyze cellulose-containing substrates more effectively. The yield of reducing sugars (RS) in the case of MCC hydrolysis after 24 h with a cellulose complex containing cellobiohydrolase I, β-glucosidase, and EGII-CBM in comparison with a complex containing EGII without CBM increased by 22%, while for chopped aspen wood the RS increased by 42%.

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REFERENCES

  1. Kumar, R., Singh, S., and Singh, O.V., J. Ind. Microbiol. Biotechnol., 2008, vol. 35, no. 5, p. 377.

    Article  CAS  Google Scholar 

  2. Merino, S.T. and Cherry, J., Adv. Biochem. Eng./Biotechnol., 2007, vol. 108, p. 95.

    CAS  Google Scholar 

  3. Sims, R.E.H., Mabee, W., Saddler, J.N., and Taylor, M., Bioresour. Technol., 2010, vol. 101, p. 1570.

    Article  CAS  Google Scholar 

  4. Tseng, C.W., Ko, T.P., Guo, R.T., Huang, J.W., Wang, H.C., Huang, C.H., Xheng, Y.S., Wang, A., and Liu, J.R., Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun., 2011, vol. 67, no. 10, p. 1189.

    Article  CAS  Google Scholar 

  5. Morozova, V.V., Gusakov, A.V., Andrianov, R.M., Pravilnikov, A.G., Osipov, D.O., and Sinitsyn, A.P., Biotechnol. J., 2010, vol. 5, p. 871.

    Article  CAS  Google Scholar 

  6. Lee, T.M., Farrow, M.F., Arnold, F.H., and Mayo, S.L., Protein Sci., 2011, vol. 20, no. 11, p. 1935.

    Article  CAS  Google Scholar 

  7. Volkov, P.V., Rozhkova, A.M., Pravil’nikov, A.G., Andrianov, R.M., Dotsenko, G.S., Bekkarevich, A.O., Koshelev, A.V., Okunev, O.N., Zorov, I.N., and Sinitsyn, A.P., Appl. Biochem. Microbiol., 2012, vol. 48, no. 1, p. 66.

    Article  CAS  Google Scholar 

  8. Sinitsyn, A.P. and Rozhkova, A.M., in Microbiology Monographs, Heidelberg: Springer, 2015, p. 1.

    Google Scholar 

  9. Aslanidis, C. and de Jong, J.P., Nucleic Acids Res., 1990, vol. 18, p. 6069.

    Article  CAS  Google Scholar 

  10. Sanger, F., Nicklen, S., and Chase, A.R., Proc. Natl. Acad. Sci. U. S. A., 1977, vol. 74, p. 5463.

    Article  CAS  Google Scholar 

  11. Aleksenko, A.Y., Makarova, N.A., Nikolaev, I.V., and Clutterbuch, A.J., Curr. Genet., 1995, no. 28, p. 474.

  12. James, P., Proteome Research: Mass Spectrometry—Principles and Practice, Heidelberg: Springer, 2001.

    Book  Google Scholar 

  13. Sinitsyn, A.P., Chernoglazov, V.M., and Gusakov, A.V., Research methods and properties of cellulolytic enzymes, Itogi Nauki Tekh., Ser.: Biotekhnol., Moscow: VINITI, 1990, vol. 25, p. 30.

    Google Scholar 

  14. Vlasenko, E., Schulein, M., Cherry, J., and Xu, F., Bioresour. Technol., 2010, vol. 101, p. 2405.

    Article  CAS  Google Scholar 

  15. Chemistry, Biochemistry and Biology of (1–3)-β-Glucans and Related Polysaccharides, Bacic, A., Fincher, G., and Stone, B., Eds., New York: Elsevier, 2019.

    Google Scholar 

  16. Dotsenko, A.S., Gusakov, A.V., Volkov, P.V., Rozhkova, A.M., and Sinitsyn, A.P., Biotechnol. Bioeng., 2016, vol. 113, p. 283.

    Article  CAS  Google Scholar 

  17. Merzlov, D.A., Zorov, I.N., Dotsenko, G.S., Denisenko, Yu.A., Rozhkova, A.M, Satrutdinov, A.D., Rubtsova, E.A., Kondrat’eva, E.G., and Sinitsyn, A.P., Biochemistry (Moscow), 2015, vol. 80, no. 4, p. 473.

    Article  CAS  Google Scholar 

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Funding

This work was partly supported by the Russian Foundation for Basic Research, project no. 18-54-80027.

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Authors and Affiliations

  1. Federal Research Centre Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, Russia

    O. G. Korotkova, M. V. Semenova, E. A. Rubtsova, E. G. Kondrat’eva, A. M. Rozhkova & A. P. Sinitsyn

  2. Department of Chemical Enzymology, Moscow State University, Moscow, Russia

    O. A. Sinitsyna & A. P. Sinitsyn

  3. Faculty of Biology, Moscow State University, Moscow, Russia

    N. M. Bibikov

Authors
  1. O. G. Korotkova
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  2. M. V. Semenova
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  3. E. A. Rubtsova
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  4. O. A. Sinitsyna
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  5. E. G. Kondrat’eva
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  6. N. M. Bibikov
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  7. A. M. Rozhkova
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  8. A. P. Sinitsyn
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Corresponding author

Correspondence to A. M. Rozhkova.

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Translated by Sh. Galyaltdinov

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Korotkova, O.G., Semenova, M.V., Rubtsova, E.A. et al. Comparative Analysis of the Properties of Recombinant Endo-1,4-β-Glucanase II and Its Chimeric Form with a Cellular Binding Module. Moscow Univ. Chem. Bull. 74, 223–228 (2019). https://doi.org/10.3103/S0027131419050067

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  • DOI: https://doi.org/10.3103/S0027131419050067

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