Abstract
The polypeptide release factor gene, eRF1, of Blepharisma japonicum (Bj-eRF1) was cloned and sequenced. Its coding region was 1314 base pairs and encodes a protein of 437 amino acids. The cloned gene was expressed in Escherichia coli and the recombinant Bj-eRF1 polypeptide was purified by Ni2+-nitrilotriacetic acid agarose and Superose12 chromatography. Pull-down analysis showed that the recombinant Bj-eRF1 interacts with the heterologously-expressed release factor, eRF3C, of Euplotes octocarinatus.
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Wang, W., Chai, Bf., Heckmann, K. et al. Cloning, characterization and expression of the polypeptide release factor gene, eRF1, of Blepharisma japonicum . Biotechnology Letters 26, 959–963 (2004). https://doi.org/10.1023/B:BILE.0000030039.42124.36
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DOI: https://doi.org/10.1023/B:BILE.0000030039.42124.36