Abstract
The Cercospora kikuchii cercosporin export gene, CFP, introduced into Beta vulgarisL. by conjugation with Rhizobium radiobacter, was stably maintained during vegetative propagation as verified by PCR using primers specific for the CFP gene. Transcriptional expression of the CFP gene in leaves was determined by RT-PCR using CFP-specific primers. CFP protein was detected using Western analysis with an affinity-purified polypeptide-specifc antibody. Analysis of the relative susceptibility of CFP-transgenic and non-transgenic sugar beet plants is planned but will probably take several years to complete.
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Kuykendall, L.D., Upchurch, R.G. Expression in sugar beet of the introduced cercosporin toxin export (CFP) gene from Cercospora kikuchii, the causative organism of purple seed stain in soybean. Biotechnology Letters 26, 723–727 (2004). https://doi.org/10.1023/B:BILE.0000024096.22105.c3
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DOI: https://doi.org/10.1023/B:BILE.0000024096.22105.c3