Abstract
A domain of epiglucan was synthesized by β-glucosidases. Two β-glucosidases, an extracellular β-glucosidase derived from Sclerotinia sclerotiorum grown on xylose, and a commercial lyophilized preparation of β-glucosidase from Aspergillus niger, were used to synthesize gluco-oligosaccharides from cellobiose and, specially, β-(1-6) branched β-(1-3) gluco-oligosaccharides, corresponding to the structure of epiglucan. Gentiobiose, cellotriose, cellotetraose, β-Glc-(1-3)-β-Glc-(1-4)-Glc, β-Glc-(1-6)-β-Glc-(1-4)-Glc and β-Glc-(1-6)-β-Glc-(1-3)-Glc were synthesized from cellobiose by both enzymes. The latter compound was preferentially synthesized by the β-glycosidase from Sclerotinia sclerotiorum. Under the best conditions, only 7 g l−1 of β-Glc-(1-6)-β-Glc-(1-3)-Glc was synthesized by the β-glycosidase from Aspergillus niger compared to 20 g l−1 synthesized with β-glycosidase from Sclerotinia sclerotiorum.
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Smaali, M.I., Michaud, N., Marzouki, N. et al. Comparison of two β-glucosidases for the enzymatic synthesis of β-(1-6)-β-(1-3)-gluco-oligosaccharides. Biotechnology Letters 26, 675–679 (2004). https://doi.org/10.1023/B:BILE.0000023029.81600.91
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DOI: https://doi.org/10.1023/B:BILE.0000023029.81600.91