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Transcription level of granule-associated phaP and phaR genes and granular morphogenesis of poly-β-hydroxyalkanoate granules in Ralstonia eutropha

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Abstract

The transcription levels of the granule-associated phaP and phaR genes in Ralstonia eutropha were regu- lated through the transformation of the phbC genes from R. eutropha and Alcaligenes latus into the poly-β-hydroxybutyrate synthase-negative mutant. The granular morphogenesis of short chain length, poly-β-hydroxyalkanoate (scl-PHA) was closely associated with the mRNA transcription levels of the phaP and phaR genes, especially with the ratio of phaP/phaR genes. The phasin protein encoded by the phaP gene increased the number of granules, while the PhaR protein of the phaR gene enlarged the size of the scl-PHA granules in R. eutropha.

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Authors and Affiliations

  1. Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu o702-701, S. Korea

    Min-Chul Seo, Hyun-Dong Shin & Yong-Hyun Lee

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  1. Min-Chul Seo
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  2. Hyun-Dong Shin
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  3. Yong-Hyun Lee
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Seo, MC., Shin, HD. & Lee, YH. Transcription level of granule-associated phaP and phaR genes and granular morphogenesis of poly-β-hydroxyalkanoate granules in Ralstonia eutropha . Biotechnology Letters 26, 617–622 (2004). https://doi.org/10.1023/B:BILE.0000023018.00625.c4

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  • DOI: https://doi.org/10.1023/B:BILE.0000023018.00625.c4

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