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Production of xylitol from Candida tropicalis by using an oxidation-reduction potential-stat controlled fermentation

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Abstract

An on-line device, ORP (oxidation-reduction potential)-stat, was used to control glucose-feeding for enhancing xylitol conversion from d-xylose during an oxygen-limited fermentation by Candida tropicalis. The fermentation was carried out in a 5 l jar fermenter. After glucose in the medium was depleted, a switching to a limited aeration and feeding glucose controlled by ORP-stat was performed. The maximum xylitol yield was obtained under a condition at an ORP of −180 mV and at an aeration rate of 0.2 l min−1.

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References

  • Cao NJ, Tang R, Gong CS, Chen LF (1994) The effect of cell density on the production of xylitol from D-xylose by yeast. Appl. Biochem. Biotechnol. 45–46: 515–519.

    Google Scholar 

  • Delgene JP, Moletta R, Navarro JM (1988) Fermentation of D-xylose, D-glucose, L-arabinose mixture by Pichia stipitis Y-7124: sugar tolerance. Appl. Microbiol. Biotechnol. 29: 155–161.

    Google Scholar 

  • Du Preez JC, van Driessel B, Prior BA (1988) The relation between redox potential and D-xylose fermentation by Candida shehatae and Pichia stipitis. Biotechnol. Lett. 10: 901–906.

    Google Scholar 

  • Emodi A (1978) Xylitol: its properties and food applications. Food Technol. 1: 28–32.

    Google Scholar 

  • Furlan SA, Boutllound P, Sterehaiano P, Iba JP (1991) Study of xylitol formation from xylose under oxygen limiting conditions. Biotechnol. Lett. 13: 203–206.

    Google Scholar 

  • Hahn-Hagerdal B, Jeppsson H, Skoog K, Prior BA (1994) Biochemistry and physiology of xylose fermentation by yeasts. Enzyme Microb. Technol. 16: 993–943.

    Google Scholar 

  • Kim SY, Kim JH, Oh DK (1997) Improvement of xylitol production by controlling oxygen supply in Candida parapsilosis. J. Ferment. Bioeng. 83: 267–270.

    Google Scholar 

  • Mäkinen KK (1992) Dietary prevention of dental caries by xylitol – clinical effectiveness and safety. J. Appl. Nutr. 44: 16–28.

    Google Scholar 

  • Melaja A, Hamalainen L (1977) Process for Making Xylitol. US Patent 4,008,285.

  • Oh DK, Kim SY, Kim JH (1998) Increase of xylitol production rate by controlling redox potential in Candida parapsilosis. Biotechnol. Bioeng. 58: 440–444.

    Google Scholar 

  • Parajó JC, Domínguez H, Domínguez JM (1998a) Biotechnological production of xylitol. Part 1: Interest of xylitol and fundamentals of its biosynthesis. Bioresour. Technol. 65: 191–201.

    Google Scholar 

  • Parajó JC, Domínguez H, Domínguez JM (1998b) Biotechnological production of xylitol. Part 2: Operation in culture media with commercial sugars. Bioresour. Technol. 65: 203–212.

    Google Scholar 

  • Pepper T, Olinger PM(1988) Xylitol in sugar-free confections. Food Technol. 10: 98–106.

    Google Scholar 

  • Tarvares JM, Duarte LC, Amaral-Collaço MT, Gírio FM (2000) The influence of hexoses addition on the fermentation of D-xylose in Debaryomyces hansenii under continuous cultivation. Enzyme Microb. Technol. 26: 743–747.

    Google Scholar 

  • Vandeska E, Amartey S, Kuzmanova S, Jeffries TW(1995a) Effects of environmental conditions on production of xylitol by Candida boidinii W.J. Microbiol. Biotechnol. 11: 213–218.

    Google Scholar 

  • Vandeska E, Kuzmanova S, Jeffries TW (1995b) Xylitol formation and key enzyme activities in Candida boidinii under different oxygen transfer rates. J. Ferment. Bioeng. 80: 513–516.

    Google Scholar 

  • Yahashi Y, Horitsu H, Kawai K, Suzuki T, Takamizawa K (1996) Production of xylitol from D-xylose by Candida tropicalis: the effect of D-glucose feeding. J. Ferment. Bioeng. 81: 148–152.

    Google Scholar 

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Sheu, DC., Duan, KJ., Jou, SR. et al. Production of xylitol from Candida tropicalis by using an oxidation-reduction potential-stat controlled fermentation. Biotechnology Letters 25, 2065–2069 (2003). https://doi.org/10.1023/B:BILE.0000007067.37858.69

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  • DOI: https://doi.org/10.1023/B:BILE.0000007067.37858.69

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