Abstract
Mitochondrial DNA (mtDNA) hypervariable segment I sequences (HVSI, 471 bp) of the control region and partial cytochrome b sequences (Cytb, 403 bp) were analyzed in three tentative species of the genus Mystacoleucus in China (M. chilopterus, M. marginatus, and M. lepturus). Not more than two mutations were found in both the HVSI and Cytb fragments among the samples from M. chilopterus and M. marginatus. However, M. lepturus differed from each of them by at least 25 mutations in Cytb and 51 mutations in HVSI. Moreover, the HVSI sequence variation within M. lepturus was larger than that between M. chilopterus and M. marginatus. Given that M. chilopterus and M. marginatus are very similar in morphology, it is reasonable to consider M. chilopterus and M. marginatus as conspecific. Our results also suggest a recent radiation of M. marginatus from downstream to upstream of the Lancangjiang (Mekong) River.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bandelt, H. J., Macaulay, V., & Richards, M. (2000). Median networks: Speedy construction and greedy reduction, one simulation, and two case studies from human mtDNA. Mol. Phylogenet. Evol. 16:8–28.
Brito, R. M., Briolay, J., Galtier, N., Bouvet, Y., & Coelho, M. M. (1997). Phylogenetic relationships within genus Leuciscus (Pisces, Cyprinidae) in Portuguese fresh waters, based on mitochondrial DNA cytochrome b sequences. Mol. Phylogenet. Evol. 8:435–442.
Chang, Y. S., Huang, F. L., & Lo, T. B. (1994). The complete nucleotide sequence and gene organization of carp (Cyprinus carpio) mitochondrial genome. J. Mol. Evol. 38:138–155.
Chu, X. L., & Cui, G. H. (1989). Barbinae. In Chu, X. L., & Chen, Y. R. (eds.), The Fishes of Yunnan, China (Part I Cyprinidae), Science Press, Beijing, pp. 135–228.
Gilles, A., Lecointre, G., Miquelis, A., Loerstcher, M., Chappaz, R., & Brun, G. (2001). Partial combination applied to phylogeny of European cyprinids using the mitochondrial control region. Mol. Phylogenet. Evol. 19:468–478.
Huang, S. Y. (1979). Notes on fishes of the genus Mystacoleucus from southern Yunnan, China, with description of a new species. Acta Zootaxonom. Sin. 4:419–421.
Kumar, S., Tamura, K., Jakobsen, I. B., & Nei, M. (2001). MEGA 2.1: Molecular Evolutionary Genetics Analysis software, Arizona State University, Tempe, Arizona.
Li S. Z. (1976). New records of Chinese fishes from the Lancang River, Yunnan. Acta Zootaxonom. Sin. 22:117–118.
Nesbo, C. L., Fossheim, T., Vollestad, L. A., & Jakobsen, K. S. (1999). Genetic divergence and phylogeographic relationships among European perch (Perca fluviatilis) population reflect glacial refugia and postglacial colonization. Mol. Ecol. 8:1387–1404.
Smith, H. M. (1945). The fresh-water fishes of Siam, or Thailand. U. S. Nat. Mus. Bull. 188:126–131.
Takahashi, H., & Goto, A. (2001). Evolution of East Asian ninespine sticklebacks as shown by mitochondrial DNA control region sequences. Mol. Phylogenet. Evol. 21:135–155.
Wu, H. L., Shao, K. T., & Lai, C. F. (1999). The Sueichan Press of Taiwan, Keelung, Latin–Chinese Dictionary of Fishes Names, pp. 587.
Wu, X. W., Lin, R. D., Chen, J. X., Chen, X. L., & He, M. J. (1977). Barbinae. In Wu, X. W. (ed.), Cyprinidae Fishes of China, Shanghai Scientific and Technical Publishers, Shanghai, pp. 229–394.
Xiao, W., Zhang, Y., & Liu, H. (2001). Molecular systematics of Xenocyprinae (teleostei: cyprinidae): Taxonomy, biogeography, and coevolution of a special group restricted in East Asia. Mol. Phylogenet. Evol. 18:163–173.
Rights and permissions
About this article
Cite this article
Kong, QP., Yao, YG., Huang, SY. et al. Mitochondrial DNA Control Region and Cytochrome b Sequence Variation in the Genus Mystacoleucus Günther (Pisces: Cyprinidae: Barbinae) from China. Biochem Genet 41, 305–313 (2003). https://doi.org/10.1023/B:BIGI.0000006031.20967.08
Issue Date:
DOI: https://doi.org/10.1023/B:BIGI.0000006031.20967.08