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Spatial Distribution of Lectin Activity in Perilesional Areas of Tobacco Leaves Inoculated with Tobacco Mosaic Virus

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Abstract

The activities of lectins and peroxidase and lignin content were studied in the perilesional area of leaves in two tobacco species (Nicotiana tabacumL., cultivar Samsun NN, and N. glutinosa L.) inoculated with tobacco mosaic virus. The development of hypersensitivity response proved to be accompanied by a complex spatial and temporal distribution of lectin activity. The area 5–9 mm away from the lesion center was characterized by the highest activity of loosely bound membrane lectins eluted with 0.05% Triton X-100. In the fraction of tightly bound membrane lectins (eluted with 0.5% detergent), lectin activity decreased during the first two days but increased on day 4 after inoculation. The activity of loosely bound membrane lectins increased in the leaf areas distant from the lesion. Two-phase dynamics in the interlesional area were also observed for lectin activity in the tightly bound membrane fraction (decrease on day 2 days and increase on day 4 after inoculation) and for peroxidase activity (increase on day 2 days and decrease on day 4). The relationship between the dynamics and spatial distribution of lectins in the perilesional area and the possible involvement of these proteins in pathogen-induced changes in photosynthesis are discussed.

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  1. Tsitsin Main Botanical Garden, Russian Academy of Sciences, ul. Botanicheskaya 4, Moscow, 127276, Russia

    A. V. Babosha

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  1. A. V. Babosha
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Babosha, A.V. Spatial Distribution of Lectin Activity in Perilesional Areas of Tobacco Leaves Inoculated with Tobacco Mosaic Virus. Biology Bulletin 31, 262–267 (2004). https://doi.org/10.1023/B:BIBU.0000030147.97570.14

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  • DOI: https://doi.org/10.1023/B:BIBU.0000030147.97570.14

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