Abstract
In this study, tissue culture method for plant regeneration from immature embryos of elite Hungarian winter wheat varieties was established. The influence of the growth regulators and the concentration of macroelements in the regeneration medium and of the incubation temperature and light intensity on regeneration frequency were investigated. The most noticeable effect on regeneration frequency was achieved by simultaneously reducing both the incubation temperature to 23 °C and the concentration of macroelements in the regeneration medium to half-strength. This modification increased the average regeneration frequency from about 10–78%. Changes in the light intensity and temperature gave an average plant regeneration frequency of 83%.
Similar content being viewed by others
References
Altpeter F, Vasil V, Srivastava V, Stoger E & Vasil IK (1996) Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep. 16: 12–17
Barro F, Martin A, Lazzeri PA & Barceló P (1999) Medium optimisation for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum. Euphytica 108: 161–167
Chalupa V (1987) Temperature. In: Bonga JM & Durzan DJ (eds) Cell and Tissue Culture in Forestry, Vol. 1 (pp. 142–151). Martinus Nijhoff Publishers, Dordrecht, The Netherlands
Constabel F & Shyluk JP (1994) Initiation, nutrition, and maintenance of plant cell and tissue culture. In: Vasil IK & Thorpe TA (eds) Plant Cell and Tissue Culture, (pp. 3–15). Kluwer Academic Publishers, Dordrecht, The Netherlands
Fennell S, Bohorova N, Van Ginkel M, Crossa J & Hoisington D (1996) Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats. Theor. Appl. Genet. 92: 163–169
Fischer-Iglesias C, Sundberg B, Neuhaus G & Jones AM (2001) Auxin distribution and transport during embryonic pattern formation in wheat. Plant J. 26: 115–129
Folling L & Olesen A (2001) Transformation of wheat (Triticum aestivum L.) microspore-derived callus and microspores by particle bombardment. Plant Cell Rep. 20: 629–636
Harvey A, Moisan L, Lindup S & Lonsdale D (1999) Wheat regenerated from scutellum callus as a source of material for transformation. Plant Cell Tiss. Org. Cult. 57: 153–156
He DG, Yang YM & Scott KJ (1989) The effect of macro elements in the induction of embryogenic callus from immature embryos of wheat (Triticum aestivum L.). Plant Sci. 64: 251–258
Hughes KW (1981) In vitro ecology: exogenous factors affecting growth and morphogenesis in plant tissue cultures. Environ. Exp. Bot. 21: 281–288
Iser M, Fettig S, Scheyhing F, Viertel K & Hess D (1999) Genotype-dependent stable genetic transformation in German spring wheat varieties selected for high regeneration potential. J. Plant Physiol. 154: 509–516
Larkin PJ (1982) Sugarcane tissue and protoplast culture. Plant Cell Tiss. Org. Cult. 1: 149–164
Machii H, Mizuno H, Hirabayashi T, Li H & Hagio T (1998) Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell Tiss. Org. Cult. 53: 67–74
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497
Ozgen M, Turet M, Altiinok S & Sancak C (1998) Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes. Plant Cell Rep. 18: 331–335
Pastori GM, Wilkinson MD, Steele SH, Sparks CA, Jones HD & Parry MAJ (2001) Age-dependent transformation frequency in elite wheat varieties. J. Exp. Bot. 52: 857–863
Pellegrineschi A, Noguera LM, Skovmand B, Brito RM, Velazquez L, Salgado MM, Hernandez R, Warburton M & Hoisington D (2002) Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants. Genome 45: 421–430
Rakszegi M, Tamás C, Szűcs P, Tamás L & Bedő Z (2001) Current status of wheat transformation. J. Plant Biotechnol. 3: 67–81
Rumary C & Thorpe TA (1984) Plantlet formation in black and white spruce. I: In vitro techniques. Can. J. For. Res. 14: 10–16
Thorpe TA (1980) Organogenesis in vitro: structural, physiological and biochemical aspects. Int. Rev. Cytol. Suppl. 11: 71–111
Thorpe TA (1994) Morphogenesis and regeneration. In: Vasil IK & Thorpe TA (eds) Plant Cell and Tissue Culture (pp. 17–36). Kluwer Academic Publishers, Dordrecht, The Netherlands
Varshney A & Altpeter F (2002) Stable transformation and tissue culture response in current European winter wheats (Triticum aestivum L.). Mol. Breed. 8: 295–309
Vasil V, Castillo A, Fromm M & Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic scutellar callus. Bio-Technology 10: 667–674
Villalobos DM, Leung DWM & Thorpe TA (1984) Light-cytokinin interaction in shoot formation in cultured cotyledon explants of radiata pine. Physiol. Plant 61: 497–504
Zhang L, Rybczynski JJ, Langenberg WG, Mitra A & French R (2000) An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration. Plant Cell Rep. 19: 241–250
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tamás, C., Szűcs, P., Rakszegi, M. et al. Effect of Combined Changes in Culture Medium and Incubation Conditions on the Regeneration from Immature Embryos of Elite Varieties of Winter Wheat. Plant Cell, Tissue and Organ Culture 79, 39–44 (2004). https://doi.org/10.1023/B:TICU.0000049447.81409.ed
Issue Date:
DOI: https://doi.org/10.1023/B:TICU.0000049447.81409.ed