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Regional Distribution of Phospholipids and Polyphosphatidyl Inositides in the Rabbit's Spinal Cord

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Abstract

The plasticity of the membrane phospholipids in general and stimulated phosphoinositides turnover in particular are the subjects in a variety of neural paradigms studying the molecular mechanisms of neuronal changes under normal and pathological conditions. The regional modifiability of phospholipids (SM, PC, PS, PI, PA + DG, PE), polyphosphatidylinositides (PI, PIP, PIP2) and diacylglycerol-dependent incorporation of CDP-choline into phosphatidylcholine in the gray matter, white matter, dorsal horns, intermediate zone and ventral horns of the rabbit's spinal cord was studied. We have found 1. a significant increase in the concentration of SM, PC, PS, DG + PA and PE in the white matter in comparison to the gray one, 2. the highest concentration of the outer membrane leaflet-bound phospholipids in the dorsal horns and the inner membrane phospholipids in the intermediate zone in comparison to the gray matter, 3. a substantial amount of labeled polyphosphatidylinositides (poly-PIs) in the spinal cord white matter with descending order PIP > PI > PIP2, 4. similar incorporation of myo-2-[3H]inositol into all poly-PIs in ventral horns and intermediate zone, but a different, lower incorporation into PI and PIP and higher into PIP2 in the dorsal horns, 5. higher diacylglycerol-dependent incorporation of CDP-choline into PC in the regionally undivided gray matter than in the white matter taken as a whole, 6. the high proportion of diacylglycerol-dependent incorporation of CDP-choline into PC in both the ventral and dorsal horns, whereas that in the intermediate zone remained low.

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  1. Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4,6, 040 01, Košice, Slovak Republic

    Nadežda Lukáčová & Jozef Maršala

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  1. Nadežda Lukáčová
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Lukáčová, N., Maršala, J. Regional Distribution of Phospholipids and Polyphosphatidyl Inositides in the Rabbit's Spinal Cord. Neurochem Res 22, 687–692 (1997). https://doi.org/10.1023/A:1027397825584

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