Abstract
The levels of indole-acetic acid (IAA), gibberellic acid1 (GA1), trans-zeatin (Z) and trans-zeatin riboside (ZR) in seedless fruits of parthenocarpic tomato (Lycopersicon esculentum Mill. cv. Rarkuna First) were analysed using 13C6-IAA, 2H2-GA1, 2H5-Z and 2H5-ZR, as internal standards by liquid chromatography–mass spectrometry. Fruits were sampled at 6 cm in diameter (referred to as 6-cm-fruit) and 8 cm (8-cm-fruit, mature green stage) and separated into pericarps, partitions and locule tissues. The pericarps and partitions were centrifuged for the collection of apoplast (AP) solution (sap outside a cell) and symplast (SP) solution (sap within a cell). IAA concentrations of the pericarps and partitions were higher in 8-cm-fruit than in 6-cm-fruit. In the partitions, IAA concentrations of SP solution were higher than those of AP solution in both 6- and 8-cm-fruit. The SP solution of the partitions in 6-cm-fruit had the highest concentration of Z (4.6 pmol/g fresh weight) and was 2.7 times than the AP solution, while in the pericarps Z concentrations were the same level in AP and SP solution. The ZR concentration in locule tissues in 6-cm-fruit (55 pmol/g fresh weight ) was the highest of all parts. The results suggest that the sites of synthesis may be the SP of partitions for IAA and Z, and locules for ZR.
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References
Abdel-Rahman M., Thomas T.H., Doss G.J. and Howell L.1975. Changes of endogenous plant hormones in cherry tomato fruits during development and maturation. Physiol. Plant.34: 39-43.
Ahmadi M. and Baker D.A.2000. Identification and quantification of the major endogenous cytokinins in pistachio seedling. Plant Growth Regul.32: 351-357.
Bohner J. and Bangerth F.1988. Effects of fruit set sequence and defoliation on cell number, cell size and hormone levels of tomato fruits (Lycopersicon esculentum Mill.) within a truss. Plant Growth Regul.7: 141-155.
Durley R.C. and Pharis R.P.1972. Partition coefficients of 27 gibberellins. Phytochemistry11: 317-326.
El-Beltagy A.S., Patrick J.P., Hewett E.W. and Hall M.A.1976. Endogenous plant growth regulator levels in tomato fruits during development. J. Hort. Sci.51: 15-30.
Fos M., Proano K., Nuez F. and Garcia-Martinez J.L.2001. Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato. Physiol. Plant.111: 545-550.
Garcia-Martinez J.L. and Hedden P.1997. Gibberellins and fruit development. In: Tomas-Barberan F.A. and Robins R.J. (eds), Phytochemistry of Fruit and Vegetables. Oxford Sci. Publications, Heidelberg, pp. 263-285.
George W.L., Scott J.W. and Splittstoesser W.E.1984. Parthenocarpy in tomato. Hort. Rev.6: 65-84.
Gillaspy G., Ben-David H. and Gruissem W.1993. Fruits: a developmental perspective. Plant Cell5: 1439-1451.
Gustafson F.G.1939. Auxin distribution in fruits and its significance in fruit development. Amer. J. Bot.26: 189-194.
Hedden P.1993. Modern methods for the quantitative analysis of plant hormones. Ann. Rev. Plant Physiol.44: 107-129.
Kojima K., Kuraishi S., Sakurai N. and Fusao K.1993. Distribution of abscisic acid in different parts of the reproductive organs of tomato. Sci. Hort.56: 23-30.
Kojima K., Sakurai N. and Tsurusaki K.1994. IAA distribution within tomato flower and fruit. HortScience29: 1200
Kojima K.1995. Simultaneous measurement of ABA, IAA and GAs in citrus–role of ABA in relation to sink ability. JARQ29: 179-185.
Kojima K.2001. Property of HPLC column and mass spectrum of LC–MS for phytohormone analysis. JARQ35: 149-154.
Kojima K., Ohtake E. and Yu Z.2002. Distribution and transport of IAA in tomato plants. Plant Growth Regul.37: 249-254.
Koshioka M., Nishijima T., Yamazaki H., Liu Y., Nonaka M. and Mander L.N.1994. Analysis of gibberellins in growing fruits of Lycopersicon esculentum after pollination or treatment with 4-chlorophenoxyacetic acid. J. Hort. Sci.69: 171-179.
Kuraishi S., Tasaki K., Sakurai N. and Sadatoku K.1991. Changes in levels of cytokinins in etiolated squash seedlings after illumination. Plant Cell Physiol.32: 585-591.
Letham D.S. and Palni L.M.S.1983. The biosynthesis and metabolism of cytokinins. Ann. Rev. Plant Physiol.34: 163-197.
Letham D.S.1987. Cytokinins. In: Letham D.S., Goodwin P.B. and Higgins T.J.V. (eds), Phytohormones and Related Compounds–a Comprehensive Treatise. Elsevier/North Holland. Amsterdam, pp. 205-264.
Laloue C., Pethe-Terrine C. and Gueern J.1981. Uptake and metabolism of cytokinin in tobacco cells. In: Guern J. and Peaud-Lenoel C. (eds), Metabolism and Molecular Activities of Cytokinins. Springer-Verlag, Berlin, Heidelberg, pp. 80-96.
Naylor A.W.1984. Functions of hormones at the organ level of organization. In: Scott T.K. (ed.), Hormonal Regulation of Development II. Encyclopedia of Plant Physiology. New seriesVol. 10. Springer-Verlag, Berlin, Heidelberg, pp. 195-200.
Nitsch J.P.1952. Plant hormones in the development of fruits. Quart. Rev. Biol.27: 33-57.
Nitsch J.P.1971. Perennation through seeds and other structures: Fruit development. In: Stewart F.C. (ed.), Plant Physiology, Vol. VI. Academic Press, New York, pp. 413-501.
Philouze J. and Maisonneuve B.1978. Heredity of the natural ability to set parthenocarpic fruits in the Soviet variety. Severianin. Tomato Genet. Coop.28: 12-13.
Sjut V. and Bangerth F.1982/83. Induced parthenocarpy–a way of changing the levels of endogenous hormones in tomato fruits (Lycopersicon esculentum Mill.) 1. Extractable hormones. Plant Growth Regul.1: 243-251.
Sjut V. and Bangerth F.1984. Induced parthenocarpy–a way of manipulating levels of endogenous hormones in tomato fruits (Lycopersicon esculentum Mill.) 2. diffusible hormones. Plant Growth Regul.2: 49-56.
Soejima H., Sugiyama T. and Ishihara K.1992. Changes in cytokinin activities and mass spectrometric analysis of cytokinins in root exudates of rice plant (Oryza sativa L.). Plant Physiol.100: 1724-1729.
Sugahara S., Sakamori M. and Aoyagi M.1995. Incorporation of parthenocarpy to greenhouse tomato cultivar III; Breeding of parthenocarpic tomato cultivar-Rarkuna First-. Res. Bull. Aichi Agric. Res. Ctr.27: 167-173. In Japanese with English summary
Takagi M., Yokota T., Murofushi N., Saka H. and Takahashi N.1989. Quantitative changes of free-base, riboside, ribotide and glucoside cytokinins in developing rice grains. Plant Growth Regul.8: 349-364.
Tsurusaki K., Masuda Y. and Sakurai N.1997. Distribution of indole-3-acetic acid in the apoplast and symplast of squash (Cucurbita maxima) hypocotyls. Plant Cell Physiol.38: 352-356.
Varga A. and Bruinsma J.1976. Roles of seeds and auxins in tomato fruit growth. Z. Pflanzenphysiol. Bd.80: 95-104.
Venis M.A., Thomas E.W., Barbier-Brygoo H., Ephritikhine G. and Guern J.1990. Impermeant auxin analogues have auxin activity. Planta182: 232-235.
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Kojima, K., Tamura, Y., Nakano, M. et al. Distribution of indole-acetic acid, gibberellin and cytokinins in apoplast and symplast of parthenocarpic tomato fruits. Plant Growth Regulation 41, 99–104 (2003). https://doi.org/10.1023/A:1027381210040
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DOI: https://doi.org/10.1023/A:1027381210040