Abstract
In vitro regeneration of Melia azedarach L. was studied. Shoots were regenerated from calli initiated from leaflets of in vitro growing plants. The best medium for establishment of cultures was Murashige and Skoog (MS) medium with 4.44 μM benzylaminopurine (BAP) + 0.46 μM kinetin (KIN) + 16.29 μM adenine sulphate (ADE). Regenerated shoots were multiplied in MS + 0.44 μM BAP + 0.37 μM KIN + 3.26 μM ADE. Maximal rooting of 89 % was achieved by culture of regenerated shoots in MS + 12.26 μM indole-3-butyric acid for 3 d and subsequently in MS lacking growth regulators for 27 d. Rooted shoots were acclimatized and successfully transferred to soil.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdullah, A.A., Yeoman, M.M., Grace, J.: Calabrian pine (Pinus brutia Tenore).-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 5, Trees II. Pp. 507-525. Springer-Verlag, Berlin 1989.
Ahmad, Z., Zaidi, N., Shah, F.H.: Micropropagation of Melia azedarach from mature tissue.-Pakistan J. Bot. 22: 172-178, 1990.
Babber, S., Mittal, K., Ahlawat, R., Varghese, T.M.: Micropropagation of Cardiospermum halicacabum.-Biol. Plant. 44: 603-606, 2001.
Baraldi, R., Rossi, F., Lercari, B.: In vitro shoot development of Prunus GF 655-2: interaction between light and benzyladenine.-Physiol. Plant. 74: 440-443, 1988.
Bordón, Y., Guardiola, J.L., García-Luis, A.: Genotype affects the morphogenetic response in vitro of epicotyl segments of Citrus rootstocks.-Ann. Bot. 86: 159-166, 2000.
Cheng, T.Y.: Factors affecting adventitious bud formation of cotyledon culture of Douglas fir.-Plant Sci. Lett. 9: 179-187, 1977.
Coleman, G.D., Ernst, S.G.: In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype.-Plant Cell Rep. 8: 459-462, 1989.
Domecq, C.: Cultivo in vitro de yemas axilares de paraíso gigante (Melia azedarach L. var. gigantea.-Phyton 48: 33-42, 1988.
Dorée, M., Leguay, J.J., Terrine, C., Sadorge, P., Trapy, F., Guern, J. Adaptation a l'adénine exogène.-In: Colloques Internacionaux C.N.R.S. No 193. Les Cultures de Tissus de Plantes. Pp. 345-365. Centre National de la Recherche Scientifique, Paris 1971.
Eeswara, J.P., Stuchbury, T., Allan, E.J., Mordue (Luntz), A.J.: A standard procedure for the micropropagation of the neem tree (Azadirachta indica A. Juss).-Plant Cell Rep. 17: 215-219, 1998.
Evans, D.A., Sharp, W.R., Flick, C.E.: Growth and behaviour of cell cultures: embryogenesis and organogenesis.-In: Thorpe, T.A. (ed.): Plant Tissue Culture. Methods and Applications in Agriculture. Pp. 45-113. Academic Press, New York 1981.
Gonzalez, A.M., Cristóbal, C.L.: Anatomía y ontogenia de semillas de Helicteres Lhotzkyana (Sterculiaceae).-Bonplandia 9: 287-294, 1997.
Johansen, D.A.: Plant Microtechnique.-McGraw Hill Book Co., New York 1940.
Kool, L.T., Keng, C.L., Hoe, C.T.K.: In vitro rooting of sentang shoots (Azadirachta excelsa L.) and acclimatization of the plantlets.-In Vitro cell. dev. Biol. Plant 35: 396-400, 1999.
Lercari, B., Tognoni, F., Anselmo, G., Chapel, D.: Photocontrol of in vitro bud differentiation in Saintpaulia ionantha leaves and Lycopersicon esculentum cotyledons.-Physiol. Plant. 67: 340-344, 1986.
Luque, R., Sousa, H.C., Kraus, J.E.: Métodos de coloracão de Roeser (1972) e Kropp (1972) visando a subtituicão do azul do astra por azul de alciao 8GS ou 8GX.-Acta bot. bras. 10: 199-212, 1996.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962.
Skoog, F.: Aspects of growth factors interactions in morphogenesis of tobacco tissue cultures.-In: Colloques Internationaux C.N.R.S. No 193. Les Cultures de Tissus de Plantes. Pp. 115-135. Centre National de la Recherche Scientifique, Paris 1971.
Skoog, F., Tsui, C.: Chemical control of growth and bud formation in tobacco stem segments and callus cultured in vitro.-Amer. J. Bot. 35: 782-787, 1948.
Thakur, R., Rao, P.S., Bapat, V.A.: In vitro plant regeneration in Melia azedarach L.-Plant Cell Rep. 18: 127-131, 1998.
Thorpe, T.A.: Organogenesis in vitro structural, physiological and biochemical aspects.-Rev. Cytol. 11A: 71-111, 1980.
Thorpe, T.A., Harry, I.S., Kumar, P.P.: Application of micropropagation to forestry.-In: Debergh, P.C., Zimmerman, R.H. (ed.): Micropropagation. Pp. 311-336. Kluwer Acad. Publ., Dordrecht 1991.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vila, S., Gonzalez, A., Rey, H. et al. In vitro Plant Regeneration of Melia azedarach L.: Shoot Organogenesis from Leaf Explants. Biologia Plantarum 47, 13–19 (2003). https://doi.org/10.1023/A:1027364427795
Issue Date:
DOI: https://doi.org/10.1023/A:1027364427795