Abstract
Influence of boron on somatic embryogenesis in papaya (Carica papaya L.) cv. Honey Dew was investigated. Immature zygotic embryos were grown in the induction medium containing Murashige and Skoog basal salts, with B5 vitamins, picloram (1 mg dm−3) or 2,4-dichlorophenoxy acetic acid (2 mg dm−3) and different concentrations of boric acid (30 to 500 mg dm−3). Maximum somatic embryo initiation was observed at 62 mg dm−3 boric acid irrespective of the growth regulator used. The cotyledonary stage somatic embryos were germinated on MS basal medium devoid of growth regulators. The regenerated plantlets were hardened under greenhouse conditions and transferred to field.
This is a preview of subscription content, log in via an institution to check access.
References
Bhattacharya, J., Khuspe, S.S., Renukdas, N.N., Rawal, S.K.: Somatic embryogenesis and plant regeneration from immature embryo explant of papaya cv. Washington and Honey Dew.-Indian J. exp. Biol. 40: 624-627, 2002.
Chandel, S.R.S.: A Handbook of Agricultural Statistics.-Achal Prakashan Mandir, New Delhi 1993.
Chen, M.H., Wang, P.J., Maeda, E.: Somatic embryogenesis and plant regeneration in Carica papaya L. tissue culture derived from root explants.-Plant Cell Rep. 6: 348-351, 1987.
Fitch, M.M.M.: High frequency somatic embryogenesis and plant regeneration from papaya hypocotyl callus.-Plant Cell Tissue Organ Cult. 32: 205-212, 1993.
Fitch, M.M.M., Manshardt, R.M.: Somatic embryogenesis and plant regeneration from immature zygotic embryos of papaya (Carica papaya L.).-Plant Cell Rep. 9: 320-324, 1990.
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151-158, 1968.
Hossain, M., Rahman, S.M., Islam, R., Joarder, O.I. High efficiency plant regeneration from petiole explants of Carica papaya L. through organogenesis.-Plant Cell Rep. 13: 99-102, 1993.
Huang, F.H., Li, X.Y.: Cold storage and boric acid produced pine embryogenesis.-Agricell Rep. 23: 12, 1994.
Medora, R.S., Campbell, J.M., Mell, G.P.: Effect of media on growth of papaya callus cultures.-Z. Pflanzenphysiol. 91: 79-82, 1979.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-479, 1962.
Rajeevan, M.S., Pandey, R.M.: Lateral bud culture of papaya (Carica papaya L.) for clonal propagation.-Plant Cell Tissue Organ Cult. 6: 181-188, 1986.
Reuveni, O., Shlesinger, D.R., Lavi, U.: In vitro clonal propagation of dioecious Carica papaya.-Plant Cell Tissue Organ Cult. 20: 41-46, 1990.
Sahasrabudhe, N.A., Nandi, M., Bahulikar, R.A.: Influence of boric acid on somatic embryogenesis of a cytosterile line of indica rice.-Plant Cell Tissue Organ Cult. 58: 73-75, 1999.
Snedecor, G.W., Cochran W.G.: Statistical Methods.-Oxford and IBH Publishing, New Delhi 1967.
Sokal, R.R., Rohlf, F.J.: Introduction to Biostatistics.-W.H. Freeman and Company, San Francisco 1973.
Winner, M.D.: Clonal propagation of papaya.-Plant Cell Tissue Organ Cult. 12: 306-310, 1988.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Renukdas, N., Mohan, M., Khuspe, S. et al. Influence of Boron on Somatic Embryogenesis in Papaya. Biologia Plantarum 47, 129–132 (2003). https://doi.org/10.1023/A:1027353520043
Issue Date:
DOI: https://doi.org/10.1023/A:1027353520043