Abstract
A procedure for in vitro propagation of dioecious papaya clones is described. A high rate of success in culture estbalishment was obtained when axillary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl-1 rifampicin or by incorporating it at 50 mgl-1 into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl-1 6-benzyladenine and 0.1 mgl-1 naphthaleneacetic acid was used for establishment and proliferation. The addition of 160 mgl-1 adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl-1 kinetin and 0.05 mgl-1 naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0 mgl-1 indole-3-butyric acid. Commercial plots of papaya plants obtained through this procedure already exist.
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References
Allan P (1964) Papaw grown from cuttings. Farming S Afr 39: 35–40
Bastiaens L (1983) Endogenous bacteria in plants and their implication in tissue culture—a review. Med Fac Landbouwwet Rijksuniv Gent 48: 1–11
Bastiaens L, Maene L, Harbaoui Y, Van Sumere C, Van de Casteele KL, Debergh PC (1983) The influence of antibacterial products on plant tissue cultures. Meded Fac Landbouwwet Rijksuniv Gent 48: 13–24
Cohen D, Cooper P (1982) Micropropagation of babaco Carica hybrid from Ecuador. Proc 5th Int Congr Plant Tissue Cell Culture, Tokyo (pp 743–744)
Debergh PC, Maene LJ (1981) A scheme for commercial propagation of ornamental plants by tissue culture. Sci Hortic 14: 335–345
De Winnaar WD (1988) Clonal propagation of papaya in vitro. Plant Cell Tissue Organ Culture 12: 305–310
Drew RA (1987) The effect of medium composition and cultural conditions on in vitro root initiation and growth of papaya (Carica papaya L.). J Hortic Sci 62: 551–556
Drew RA, Smith NG (1986) Growth of apical and lateral buds of papaw (Carica papaya L.) as affected by nutritional and hormonal factors. J Hortic Sci 61: 535–543
Falcone AM, Leva AR (1986) Moltiplicazione in vitro della Carica papaya L. Riv di Agric Subtrop e Trop 80 71–79
Litz RE, Conover RA (1977) Tissue culture propagation of papaya. Proc Fla State Hortic Soc 90: 245–246
Litz RE, Conover RA (1978) Recent advances in papaya tissue culture. Proc Fla State Hortic Soc 91: 180–182
Litz RE, Conover RA (1978) In vitro propagation of papaya. HortScience 13: 241–242
Litz RE, Conover RA (1981) Effect of sex type, season and other factors on in vitro establishment and culture of Carica papaya L. explants. J Am Soc Hortic Sci 106: 792–794
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Phillips R, Arnott SM, Kaplan SE (1981) Antibiotics in plant tissue culture: Rifampicin effectively controls contaminants without affecting the growth of short-term explant cultures of Helianthus tuberosus. Plant Sci Lett 21: 235–240
Rajeevan MS, Pandey RM (1986) Lateral bud culture of papaya (Carica papaya L.) for clonal propagation. Plant Cell Tissue Organ Culture 6: 181–188
Soomark S, Tai EA (1975) Vegetative propagation of papaya by budding. Acta Hortic 49: 85–90
Yie ST, Liaw SI (1977) Plant regeneration from shoot tips and callus of papaya. In Vitro 13: 564–568
Young PM, Hutchins AS & Canfield MC (1984) Use of antibiotics to control bacteria in shoot cultures of woody plants. Plant Sci Lett 34: 203–209
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Reuveni, O., Shlesinger, D.R. & Lavi, U. In vitro clonal propagation of dioecious Carica papaya . Plant Cell Tiss Organ Cult 20, 41–46 (1990). https://doi.org/10.1007/BF00034755
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DOI: https://doi.org/10.1007/BF00034755