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Linkage Mapping of DNA Markers Generated with Specific and Non-Specific Gene Primers in Soybean

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Biologia Plantarum

Abstract

Polymerase chain reaction (PCR) has been used extensively in the construction of linkage maps for many cultivated crops including soybean, [Glycine max (L.) Merr]. In this study, four sets of oligonucleotide primer pairs of known genes (pearl millet Adh 1, nodule specific proline-rich protein, Drosophila homeobox, heat shock protein), several different combinations from kits A, D, E, and J of arbitrary primers and five primer pairs of soybean simple sequence repeats of varying length (Satt 9, Satt 20, Satt 42, Satt 64, and Satt 30) were utilized in PCR to identify molecular markers which were then used to construct a genetic linkage map. DNA for the PCR reactions was isolated from 65 recombinant inbred soybean lines resulting from crossing PI 290,136 and BARC-2 (Rj 4 ), followed by self-pollination for seven generations without selection. Mapmaker 3.0, a computer package, was used for construction of the linkage map. A total of 43 polymorphic markers were identified; 30 markers were linked and distributed among 5 linkage groups while 13 markers were unlinked. Arbitrary primers revealed more polymorphisms than specific primers. A combination of arbitrary primers A5 and A18 revealed the maximum number of polymorphic bands. Five observed linkage groups can be expanded in future soybean research by using additional markers.

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Authors and Affiliations

  1. Department of Plant and Soil Science, Alabama A&M University, Normal, AL, 35762, USA e-mail

    K.M. Soliman & M. Paladugu

  2. Weed Science Laboratory, PSI, BLDG 001, BARC-W, USDA/ARS, Beltsville, MD, 20705, USA

    T.E. Devine

Authors
  1. K.M. Soliman
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  2. M. Paladugu
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  3. T.E. Devine
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Correspondence to K.M. Soliman.

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Soliman, K., Paladugu, M. & Devine, T. Linkage Mapping of DNA Markers Generated with Specific and Non-Specific Gene Primers in Soybean. Biologia Plantarum 43, 337–346 (2000). https://doi.org/10.1023/A:1026703823512

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