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Direct Quantitative Evaluation of the Effects of Biocides on Pseudomonas fluorescens in Various Nutrient Media

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Abstract

A method for quantitative evaluation of the effects of biocides is presented. The method was tested in experiments with Pseudomonas fluorescens grown on various agar nutrient media. The effective concentrations of biocides that decreased the maximum specific rate of the colony biomass growth (μ"m) were called S (suppressing) concentrations, and concentrations that decreased the number of colony-forming units (CFU) were taken as L (sublethal) concentrations. The efficiency of the reported approach was demonstrated in experiments with three biocides tested in four nutrient media. It was found that the biocide sensitivity of Pseudomonas fluorescens varied by a factor of 30, depending on the amount and type of the nutrient substrate.

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Authors and Affiliations

  1. State Research Center for Applied Microbiology, Obolensk, Moscow oblast, 142279, Russia

    V. B. Rodin, S. K. Jigletsova, N. A. Akimova & V. P. Kholodenko

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  1. V. B. Rodin
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  2. S. K. Jigletsova
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  3. N. A. Akimova
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  4. V. P. Kholodenko
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Rodin, V.B., Jigletsova, S.K., Akimova, N.A. et al. Direct Quantitative Evaluation of the Effects of Biocides on Pseudomonas fluorescens in Various Nutrient Media. Applied Biochemistry and Microbiology 36, 609–612 (2000). https://doi.org/10.1023/A:1026656925603

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