A Qualitative Assessment of Neuron Populations Expressing the Enzymes of Dopamine Synthesis in the Rat Accurate Nucleus during Ontogeny

Abstract

The ratio of neuron populations expressing either tyrosine hydroxylase or aromatic L-amino acid decarboxylase, which are enzymes of dopamine synthesis, was estimated quantitatively in the accurate nucleus of male and female rats on the 21st day of intrauterine development, the 9th day of postnatal development, and in adult animals. The enzymes in neurons were revealed by double immunocytochemical labeling, followed by identification under a fluorescence microscope. At all the developmental stages, three neuron populations differing in the expression of these enzymes were revealed. By the end of the prenatal period, most of the neurons (99%) contained only one of the enzymes, and the proportion of neurons expressing both enzymes (dopaminergic neurons) did not exceed 1%. During postnatal development, the proportion of neurons with one enzyme proved to decrease, whereas that of dopaminergic neurons increased. However, the latter proportion, even in adult animals, did not exceed 50% of the total number of neurons expressing the enzymes of dopamine synthesis. Thus, the population of neurons expressing both enzymes increases during rat ontogeny, whereas the number of neurons expressing only one enzyme decreases.