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Pear mutagenesis: In vitro treatment with gamma-rays and field selection for productivity and fruit traits

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Abstract

In vitro shoots of six pear (Pyrus communis L.) cultivars, ‘Conference’, ‘Doyennéd'Hiver’, ‘Passe Crassane’, ‘Bartlett’, ‘AbbéFetel’ and ‘Butirra Precoce Morettini’ were irradiated with gamma rays (3.5 Gy). After three subcultures, microcuttings from the irradiated shoots and from additional non-irradiated microcuttings were rooted to establish plants for survey orchards. All trees were individually observed for variation in fruit traits and for productivity. Trees were selected for improved characters related to production such as early bearing and consistent productivity. Variations observed in fruit appearance concerned degree of russeting, fruit shape and size. The frequencies of the observed variations in fruit traits depended on the cultivar, ranging from 0.81% in ‘Doyennéd'Hiver’ to 3.64% in ‘Passe Crassane’. Of the 97 variants selected, only two showed chimeral behavior.

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References

  • Ahloowalia, B.S., 1998 In-vitro techniques and mutagenesis for the improvement of vegetatively propagated plants. In: M.S. Jain, D.S. Brar & B.S. Ahloowalia (Eds.), Somaclonal Variation and Induced Mutations in Crop Improvement, pp. 293–309. Kluwer Academic Publishers, Dordrecht, NL.

    Google Scholar 

  • Broertjes, C., 1982. Significance of in vitro adventitious bud techniques for mutation breeding of vegetatively propagated crops. In: Induced mutations in vegetatively propagated plants, II, pp. 1–9. International Atomic Energy Agency, Wien.

    Google Scholar 

  • Campbell, A.I. & T.R. Sparks, 1985. Compact mutants: production, selection and performance in ‘Bramley's Seedling' apple. Acta Hort 180: 11–18.

    Google Scholar 

  • Decourtye, L., 1970. Methodology in induced mutagenesis and results. In: Proc. Eucarpia Fruit Breed Symp Angers, pp. 161–174. Eucarpia Fruit Sect S.E.I., C.N.R.A., Versailles.

    Google Scholar 

  • Decourtye, L., 1982. Bilancio di 20 anni di miglioramento delle specie legnose da frutto mediante la mutagenesi e prospettive attuali. Atti della giornata di studio sull'uso di tecniche nucleari per il miglioramento genetico dei fruttiferi, pp. 21–40. Roma.

  • Fujimaki, H., 1996. Tangency of artificial mutation to recombinant-DNA in plant breeding. Gamma Field Symposia. The tangency of mutation induction and genetic engineering in plant breeding. July 24–25, Institute of Radiation Breeding, NIAR MAFF, Ibaraki, Japan 35: 1–4.

    Google Scholar 

  • Hirata, N., 1989. Self-compatible mutant in Japanese pear. Gamma Field Symposia. Production of mutants in tree crops. July 20–21, Institute of Radiation Breeding, NIAR MAFF, Ibaraki, Japan 28: 71–78.

    Google Scholar 

  • Lacey, C.N.D., 1975. Induction and selection of mutant form of fruit plants. Long Ashton Ann Rep 22–24.

  • Lacey, C.N.D. & I.A. Campbell, 1982. Progress in mutation breeding of apples (Malus pumila Mill.) at Long Ashton Research Station, Bristol, United Kingdom. In: Induced Mutations in Vegetatively Propagated Plants, II, pp. 11–28. International Atomic Energy Agency, Wien.

    Google Scholar 

  • Lapins, K.O., C. Bailey & L.F. Hough, 1969. Effects of gamma rays on apple and peach leaf buds at different stages of development. I. Survival, growth and mutation frequencies. Radiation Bot 9: 379–389.

    Article  Google Scholar 

  • Le Lézec, M., 1998. Qualcosa di nuovo nel regno delle pere? Frutticoltura 9(15) 16–21.

    Google Scholar 

  • Jain, M.S., 1997. Creation of variability by mutation and tissue culture for improving plants. Acta Hort 447: 69–77.

    Google Scholar 

  • Masuda, T., T. Yoshioka, K. Inoue, K. Murata, K. Kitagawa, H. Tabira, A. Yoshida, K. Kotobuki & T. Sanada, 1997. Selection of mutants resistant to black spot disease by chronic irradiation of gamma-rays in Japanese pear ‘Osanijisseiki'. J Japan Soc Hort Sci 66: 85–92.

    Article  Google Scholar 

  • Predieri, S., 1998a. Compact pears obtained through in vitro mutagenesis. Acta Hort 475: 93–98.

    Google Scholar 

  • Predieri, S., 1998b. Due nuove cultivar di pero a struttura compatta: ‘Abate Light' e ‘Conference Light'. Frutticoltura 9(15): 42–43.

    Google Scholar 

  • Predieri, S., G. Bertazza & F. Gennari, 1998. Pear cv Bartlett selection for high fruit quality: analysis of soluble sugars and organic acids. Atti IV Giornate Scientifiche SOI, Sanremo, 1–3 Aprile 1998, pp. 65–66.

  • Predieri, S., M. Magli & R.H. Zimmerman, 1997. Pear mutagenesis: in vitro treatment with gamma-rays and field selection for vegetative traits. Euphytica 93: 227–237.

    Article  Google Scholar 

  • Roby, F., 1972a. Doce mutaciones en el peral Williams obtenidas por injertos de ramitasirradiadas. Rev Invest Agropec Ser 2. 9(3): 55–64.

    Google Scholar 

  • Roby, F., 1972b. Mutaciones inducida por irradiacion en el peral Packham's Triumph. In: Induced Mutation and Plant Improvement, Buenos Aires, 1970, pp. 475–483, International Atomic Energy Agency, Vienna.

    Google Scholar 

  • Sansavini, S., 1994. Performance of micropropagated pear trees. Acta Hort 367: 260–266.

    Google Scholar 

  • SAS/INSIGHT, 1993. User's Guide, Version 6, SAS Institute Inc., Cary, NC, Fit Analyses, pp. 397–454.

    Google Scholar 

  • Spiegel-Roy, P., 1990. Economic and agricultural impact of mutation breeding in fruit trees. Mutation Breeding Rev 5: 1–26.

    Google Scholar 

  • van Harten, A.M., 1998. Mutation Breeding, Theory and Practical Applications. Cambridge Univ. Press. ISBN 0–521–47074–9.

  • Visser, T., J.J. Verhaegh & D.P. De Vries, 1971. Pre-selection of compact mutants induced by X-ray treatment in apple and pear. Euphytica 20: 195–207.

    Article  Google Scholar 

  • Yang, H.Y. & Schmidt, H., 1994. Selection of a mutant from adventitious shoots formed in X-ray treated cherry leaves and differentiation of standard and mutant with RAPDs. Euphytica 77: 89–92.

    Google Scholar 

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Predieri, S., Zimmerman, R.H. Pear mutagenesis: In vitro treatment with gamma-rays and field selection for productivity and fruit traits. Euphytica 117, 217–227 (2001). https://doi.org/10.1023/A:1026594103277

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