Abstract
The toxicity of pure phenol, formaldehyde, and industrial wastewater, containing phenol and formaldehyde, from a resin production plant was evaluated using aquatic organisms from different taxonomic groups. Test organisms included mixed bacterial culture, unicellular green algae Scenedesmus quadricauda (Turp.) Breb., crustacea Daphnia pulex de Geer (daphnids), and fish Oncorhynchus mykiss Call, 1990 (rainbow trout).
Formaldehyde was found to be more toxic to the mixed bacterial culture (120h EC50 = 34.1 mg L-1), algae (24h EC50 = 14.7 mg L-1), and crustacea (48h EC50 = 5.8 mg L-1) than phenol. Phenol proved to be more toxic to fish (48h LC50 = 13.1 mg L-1) than to the mixed bacterial culture (120h EC50 = 510 mg L-1), algae (24h EC50 = 403 mg L-1), and crustacea (48h EC50 = 25 mg L-1). The toxicity of the industrial wastewater to the mixed bacterial culture, algae, and crustacea was caused mainly by formaldehyde, but for fish the presence of phenol in the wastewater proved to be the significant reason for toxicity. Differences in sensitivity of the selected test organisms were also observed, with fish and crustacea being the most sensitive species.
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Alsop, G., Waggy, G. T. and Conway, R. A.: 1980, J. Water Pollut. Control Fed. 52, 2452.
APHA-AWWA-WEF: 1992, Standard Methods for the Examination of Water and Wastewater, 18 Edition, Washington, D.C.
Baudo, R.: 1987, Mem. Ist. Ital. Idrobiol. 45, 461.
Bitton, G. and Dutka, J. B.: 1986, Toxicity Testing using Microorganisms, vol. 1, CRC Press, Boca Raton, Florida.
Block, J. C., Bauda, P. and Reteuna, C.: 1989, 'Ecotoxicity Testing Using Aquatic Bacteria', in A. Boudou and F. Ribeyre (eds.), Aquatic Ecotoxicology: Fundamental Concepts and Methodologies, vol. II, CRC Press, Boca Raton, Florida.
Bringmann, G. and Kühn, R.: 1960, Gesundheits-Ingenieur 11, 337.
Bringmann, G. and Kühn, R.: 1980, Water Res. 14, 231.
Bringmann, G. and Kühn, R.: 1982, Z. Wasser Abwasser Forsch. 15, 1.
Curtis, C., Lima, A., Lozano, S. J. and Veith, G. D.: 1982, in Aquatic Toxicology and Hazard Assessment, Proceedings of the fifth symposium on Aquatic Toxicology, ASTM STP 766, Philadelphia, 170.
Dorward, J. E. and Barisas, B. G.: 1984, Environ. Sci. Technol. 18, 967.
Grahl, K.: 1983, Acta Hydrochimica et Hydrobiologia 11, 137.
Hunter, J. B.: 1978, Wat. Pollut. Control 77, 384.
International Organization of Standardisation: 1989, ISO Water Quality –Determination of Biochemical Oxygen Demand after 5 days (BOD5) –Dilution and Seeding Method, ISO 5815:1989(E).
International Organization of Standardization: 1989, ISO Water Quality –Determination of Chemical Oxygen Demand, ISO 6060:1989(E).
International Organization of Standardization: 1991, ISO Water Quality –Evaluation in an Aqueous Medium of the “Ultimate” Aerobic Biodegradability of Organic Compounds –Method by Determining the Oxygen Demand in a Closed Respirometer, ISO 9408:1991(E).
Leithe, W.: 1974, Die Analyse der Luft und ihrer Verunreinigungen in der freien Atmosphäre und am Arbeitsplatz, Wissenschaftliche Verlagsgesellschaft MBH, Stuttgart.
Liebmann, H.: 1960, Handbuch der Frischwasser und Abwasserbiologie, II, R. Oldenbourg, München.
McFeters, G. A., Bond, P. J., Olson, S. B. and Tchan, Y. T.: 1983, Water Res. 17, 1757.
Offhaus, K.: 1973, Münchner Beitr. Abwasser-, Fisch.-Flussbiol. 24, 169.
Organisation for Economic Cooperation and Development: 1982, Daphnia sp., Acute Immobilisation Test and Reproduction Test, 202, Paris.
Peukert, V.: 1967, Fortschr. Wasserchem. ihrer Grenzgeb. 5, 323.
Phipps, G. L., Harden, M. J., Leonard, E. N., Roush, T. H., Spehar, D. L., Stephan, C. E., Pickering, Q. H. and Buikema, A. L.: 1984, J. Water Pollut. Control Fed. 56, 725.
Qureshi, A. A., Flood, K. W., Thompson, S. R., Janhurst, S. M., Inniss, C. S. and Rokosh, D. A: 1982, Aquatic Toxicology and Hazard Assessment, Proceeding of the fifth symposium on Aquatic Toxicology, ASTM STP 766, Philadelphia, 179.
Rippen, G.: 1984, Handbuch der Umweltchemikalien, Physikalisch-chemische und ökotoxikologische Daten ausgewählter chemischer Stoffe, Ecomed, Landsberg/Lech.
Shigeoka, T., Sato, Y., Takeda, Y., Yoshida, K. and Yamauchi, F.: 1988, Environ. Toxicol. Chem. 7, 847.
Skibba, W. D.: 1982, Ausgewählte Methoden der Wasseruntersuchung, Biologische, mikrobiologische und toxikologische Methoden: Band II., VEB Gustav Fischer Verlag Jena.
Sprague, J. B.: 1969, Water Res. 3, 793.
Statistical Support Staff Computer Sciences Corporation: 1988, Users Guide for a Computer Program for Probit/s Analysis of Data from Acute and Short-term Chronic Toxicity Tests with Aquatic Organisms. The Biological Methods Branch, Environmental Monitoring and Support Laboratory, United States Environmental Protection Agency, Cincinnati.
Tümpling von, W.: 1972, Fortschr. Wasserchem. ihrer Grengeb. 14, 205.
Verschueren, K.: 1977, Handbook of Environmental Data on Organic Chemicals, Van Nostrand Reinhold Company, New York.
Vittozzi, L. and De Angelis, G.: 1991, Aquat. Toxicol. 19, 167.
Wall, T. M. and Hanmer, R. W.: 1987, J. Water Pollut. Control Fed. 59, 7.
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TIŠLER, T., ZAGORC-KONČAN, J. COMPARATIVE ASSESSMENT OF TOXICITY OF PHENOL, FORMALDEHYDE, AND INDUSTRIAL WASTEWATER TO AQUATIC ORGANISMS. Water, Air, & Soil Pollution 97, 315–322 (1997). https://doi.org/10.1023/A:1026472313561
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DOI: https://doi.org/10.1023/A:1026472313561