Skip to main content
SpringerLink
Log in

Characterization of the tissue-specific expression of the S100P gene which encodes an EF-hand Ca2+-binding protein*

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

S100 proteins are a calcium-binding protein family containing two EF-hand domains exclusively expressed in vertebrates and play roles in many cellular activities. Human S100P gene was first cloned as a 439 bp cDNA in placenta and it was found to be associated with human prostate cancer. Here we describe the cloning of the 1297 bp full-length cDNA, and the characterization of the tissue-specific expression of the human S100P gene. It is abundantly expressed in many tissues including placenta by Northern blot and RT-PCR analysis, unlike the expression pattern of other S100 family genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ali MS, Harmer M, Vaughan R (2000) Serum S100 protein as a marker of cerebral damage during cardiac surgery. Br J Anaesth. 85: 287–298.

    Google Scholar 

  2. Averboukh L, Liang L, Kantoff PW, Pardee AB (1996) Regulation of S100P expression by androgen. Prostate. 29: 350–354.

    Google Scholar 

  3. Becker T, Gerke V, Kube E, Weber K (1992) S100P, a novel Ca(2+)-binding protein from human placenta. cDNA cloning, recombinant protein expression and CA2+ binding properties. Eur J Biochem. 207: 541–547.

    Google Scholar 

  4. Berridge MJ, Lipp P, Bootman MD (2000) The calcium entry pas de deux. Science. 287: 1604–1605.

    Google Scholar 

  5. Berridge JB, Bootman, MD, Lipp P (1998) Calcium - a life and death signal. Science. 15: 645–648. Burgoyne RD, Weiss JL (2001) The neuronal calcium sensor family of Ca2+-binding proteins. Biochem J. 353: 1-12.

    Google Scholar 

  6. Chin D, Means AR (2000) Calmodulin: a prototypical calcium sensor. Trends. Cell. Bio. 10: 322–328.

    Google Scholar 

  7. Crabtree GR (2001) Calcium, calcineurin, and the control of transcription. J. Bio. Chem. 276: 2313–2316.

    Google Scholar 

  8. Donato R (2001) S100: a multigenic family of calciummodulated proteins of the EF-hand type with intracellular and 248 extracellular functional roles. Int J Biochem Cell Biol. 33: 637–668.

    Google Scholar 

  9. Donato R (1999) Functional roles of S100 proteins, calciumbinding proteins of the EF-hand type. Biochim Biophys Acta. 1450: 191–231.

    Google Scholar 

  10. Frey N, Mckinsey TA, Olson EN (2000) Decoding calcium signals involved in cardiac growth and function. Nat. Genet. 6: 1221–1227.

    Google Scholar 

  11. Gribenko A, Lopez MM, Richardson JM, Makhatadze GI (1998) Cloning, overexpression, purification, and spectroscopic characterization of human S100P. Protein Sci 7: 211–215.

    Google Scholar 

  12. Heizmann CW (1999) Ca2+-binding S100 proteins in the central nervous system. Neurochem Res. 24: 1097–1100.

    Google Scholar 

  13. Heizmann CW, Cox JA (1998) New perspectives on S100 proteins: a multi-functional (Ca(2+)-, Zn(2+)-and Cu(2+)-binding protein family. Biometals. 11: 383–397.

    Google Scholar 

  14. Heizmann CW, Fritz G, Schafer BW (2002) S100 proteins: structure, functions and pathology. Front Biosci. 7: 1356–1368.

    Google Scholar 

  15. Honore B, Vorum H (2000) The CREC family, a novel family of multiple EF-hand, low-affinity Ca2+-binding proteins localised to the secretory pathway of mammalian cells. FEBS Lett. 466: 11–18.

    Google Scholar 

  16. Kawasaki H, Nakayama S, Kretsinger RH (1998) Classi-fication and evolution of EF-hand proteins. Biometals. 11: 277–295.

    Google Scholar 

  17. Maler L, Sastry M, Chazin WJ (2002) A structural basis for S100 protein specificity derived from comparative analysis of apo and Ca(2+)-calcyclin. J Mol Biol 317: 279–290.

    Google Scholar 

  18. Mellstrom B, Naranjo JR (2001) Ca2+-dependent transcriptional repression and derepression: DREAM, a direct effector. Semin. Cell. Dev. Biol. 12: 59–63.

    Google Scholar 

  19. Otterbein LR, Kordowska J, Witte-Hoffmann C, Wang CL, Dominguez, R (2002) Crystal Structures of S100A6 in the Ca(2+)-Free and Ca(2+)-Bound States. The Calcium Sensor Mechanism of S100 Proteins Revealed at Atomic Resolution. Structure (Camb). 10: 557–567.

    Google Scholar 

  20. Sato N., Hitomi J. (2002) S100P expression in human esophageal epithelial cells: Human esophageal epithelial cells sequentially produce different S100 proteins in the process of differentiation. Anat Rec. 267: 60–9.

    Google Scholar 

  21. Schäfer BW, Wicki R, Engelkamp D, Mattei MG, Heizmann CW (1995) Isolation of a YAC clone covering a cluster of nine S100 genes on human chromosome 1q21: rationale for a new nomenclature of the S100 calcium-binding protein family. Genomics. Vol 25: 638–643.

    Google Scholar 

  22. Smith SP, Shaw GS (1998) A change-in-hand mechanism for S100 signalling. Biochem Cell Biol 76: 324–333.

    Google Scholar 

  23. Soderling TR, Chang B, Brickey D (2001) Cellular signaling through multifunctional Ca2+/calmodulin-dependent protein kinase II. J. Bio. Chem. 276: 3719–3722.

    Google Scholar 

  24. Stull J.T. (2001) Ca2+-dependent cell signaling through calmodulin-activated protein phosphatases and protein kinases. J. Bio. Chem. 276: 2311–2312.

    Google Scholar 

  25. Robinson MJ, Tessier P, Poulsom R, Hogg N (2002) The S100 family heterodimer, MRP-8/14, binds with high af-finity to heparin and heparan sulfate glycosaminoglycans on endothelial cells. J Biol Chem. 277: 3658–3665.

    Google Scholar 

Download references

Author information

Author notes

  1. Shu Wang

    Present address: Department of Genetics, School of Medicine, Yale University, 333 Cedar Street, New Haven, CT, 06520, USA

Authors and Affiliations

  1. Department of General Surgery, Changhai Hospital, Shanghai, 200433, People's Republic of China

    Gang Jin, Xiangui Hu & Zaiping Jing

  2. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China

    Shu Wang, Jinzhong Chen, Kang Ying, Yi Xie & Yumin Mao

Authors
  1. Gang Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiangui Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zaiping Jing
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jinzhong Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kang Ying
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yi Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yumin Mao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jin, G., Wang, S., Hu, X. et al. Characterization of the tissue-specific expression of the S100P gene which encodes an EF-hand Ca2+-binding protein* . Mol Biol Rep 30, 243–248 (2003). https://doi.org/10.1023/A:1026311423326

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026311423326

Search

Navigation