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Chromium (III) enhanced diamine silver staining of proteins and DNA in gels

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Abstract

Chromium (III) enhanced the sensitivities of diamine silver staining of four proteins between 6- and 50-fold over that of the Coomassie Brilliant Blue (CBB)-chromium modified thiosulfate-silver staining method (Zhou et al. Biotechnology Letters, 2002, 24: 1561–1567). Using six dsDNA fragments, the detection limits of this new method was 10 to 30 pg per band, being 10- to 25-fold more sensitive than previous methods.

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Authors and Affiliations

  1. State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, 200031, P.R. China

    Zhi-dong Zhou & Wang-yi Liu

  2. Laboratory of Nanobiology and Nanomedicine, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, 2019 Jia-Luo Road, Shanghai, 201800, P.R. China

    Zhi-dong Zhou & Min-qian Li

Authors
  1. Zhi-dong Zhou
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  2. Wang-yi Liu
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  3. Min-qian Li
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Correspondence to Wang-yi Liu.

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Zhou, Zd., Liu, Wy. & Li, Mq. Chromium (III) enhanced diamine silver staining of proteins and DNA in gels. Biotechnology Letters 25, 1801–1804 (2003). https://doi.org/10.1023/A:1026280519327

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  • DOI: https://doi.org/10.1023/A:1026280519327

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