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A genetic map of macadamia based on randomly amplified DNA fingerprinting (RAF) markers

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Abstract

The first genetic linkage map of macadamia (Macadamia integrifolia and M. tetraphylla) is presented. The map is based on 56 F1 progeny of cultivars ‘Keauhou’ and ‘A16’. Eighty-four percent of the 382 markers analysed segregated as Mendelian loci. The two-way pseudo-testcross mapping strategy allowed construction of separate parental cultivar maps. Ninety bridging loci enabled merging of these maps to produce a detailed genetic map of macadamia, 1100 cm in length and spanning 70–80% of the genome. The combined map comprised 24 linkage groups with 265 framework markers: 259 markers from randomly amplified DNA fingerprinting (RAF), five random amplified polymorphic DNA (RAPD), and one sequence-tagged microsatellite site (STMS). The RAF marker system unexpectedly revealed 16 codominant markers, one of them a putative microsatellite locus and exhibiting four distinct alleles in the cross. This molecular study is the most comprehensive examination to date of genetic loci of macadamia, and is a major step towards developing marker-assisted selection for this crop.

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References

  • Aradhya, M.K., L.K. Yee, F.T. Zee & R.M. Manshardt, 1998. Genetic variability in Macadamia. Genet Res Crop Ev 44: 1-14.

    Google Scholar 

  • Beckmann, J.S. & M. Soller, 1990. Towards a unified approach to the genetic mapping of eukaryotes based on sequence-tagged microsatellite sites. Bio/Technology 8: 930-932.

    Article  PubMed  CAS  Google Scholar 

  • Caetano-Anollés, G., B.J. Bassam & P.M. Gresshoff, 1991. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio/Technology 9: 553-557.

    Article  PubMed  Google Scholar 

  • Conner, P.J., S.K. Brown & N.F. Weeden, 1998. Molecular-marker analysis of quantitative traits for growth and development in juvenile apple trees. Theor Appl Genet 96: 1027-1035.

    Article  CAS  Google Scholar 

  • Crouzillat, D., E. Lerceteau, V. Petiard, J. Morera, H. Rodriguez, D. Walker, W. Phillips, C. Ronning, R. Schnell, J. Osei & P. Fritz, 1996. Theobroma cacao L.: a genetic linkage map and quantitative trait loci analysis. Theor Appl Genet 93: 205-214.

    Article  CAS  Google Scholar 

  • Echt, C.S. & C.D. Nelson, 1997. Linkage mapping and genome length in eastern white pine (Pinus strobus L.). Theor Appl Genet 94: 1031-1037.

    Article  CAS  Google Scholar 

  • Grattapaglia, D. & R. Sederoff, 1994. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross mapping strategy and RAPD markers. Genetics 137: 1121-1137.

    PubMed  Google Scholar 

  • Hamilton, R.A. & P.J. Ito, 1976. Development of macadamia nut cultivars in Hawaii. Calif Macadamia Soc Yrbk 22: 94-100.

    Google Scholar 

  • Hemmat, M., N.F. Weeden, A.G. Manganaris & D.M. Lawson, 1994. Molecular marker linkage map for apple. J Hered 85: 4-11.

    PubMed  CAS  Google Scholar 

  • Hulbert, S.H., T.W. Ilott, E.J. Legg, S.E. Lincoln, E.S. Lander & R.W. Michelmore, 1988. Genetic analysis of the fungus Bremia lactucae, using restriction fragment length polymorphisms. Genetics 120: 947-958.

    PubMed  CAS  Google Scholar 

  • Marques, C.M., J.A. Aradjo, J.G. Ferreira, R. Whetten, D.M. O'Malley, B.-H. Liu & R. Sederoff, 1998. AFLP genetic maps of Eucalyptus globulus and E. tereticornis. Theor Appl Genet 96: 727-737.

    Article  CAS  Google Scholar 

  • McConchie, C.A., N.M. Meyers, V. Vithanage & C.G.N. Turnbull, 1997. Pollen Parent Effects on Nut Quality and Yield in Macadamia-Final Report Project MC 302. Horticultural Research and Development Corporation, p. 19. HRDC, Australia.

    Google Scholar 

  • Meyers, N., 1997. Pollen Parent Effects on Macadamia Yield. PhD dissertation. University of Queensland, Australia.

    Google Scholar 

  • Olson, M., L. Hood, C. Cantor & D. Botstein, 1989. A common language for physical mapping of the human genome. Science 245: 1434-1435.

    PubMed  CAS  Google Scholar 

  • Paglia, G. & M. Morgante, 1998. PCR-based multiplex DNA fingerprinting techniques for the analysis of conifer genomes. Mol Breeding 4: 173-177.

    Article  CAS  Google Scholar 

  • Paterson, A.H., S.D. Tanksley & M.E. Sorrels, 1991. DNA markers in plant improvement. Adv Agron 46: 39-90.

    Article  CAS  Google Scholar 

  • Pooler, M.R. & R. Scorza, 1995. Aberrant transmission of RAPD markers in haploids, doubled haploids, and F1 hybrids of peach: observations and speculations on causes. Scientia Horticult 64: 233-241.

    Article  Google Scholar 

  • Powell, W., G.C. Machray & J. Provan, 1996a. Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1: 215-222.

    Google Scholar 

  • Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey & A. Rafalski, 1996b. Comparison of RFLP, RAPD, AFLP, and SSR (microsatellite) markers for germplasm improvement. Mol Breeding 2: 225-238.

    Article  CAS  Google Scholar 

  • Sedgley, M., F.D.H. Bell, D. Bell, C.W. Winks, S.J. Pattison & T.W. Hancock, 1990. Self-and cross compatibility of macadamia cultivars. J Hortic Sci 65: 205-218.

    Google Scholar 

  • Stace, H.M., A.W. Douglas & J.F. Sampson, 1998. Did ‘paleopolyploidy’ really occur in Proteaceae? Aus Sys Bot 11: 613-629.

    Article  Google Scholar 

  • Stam, P. & J.W. van Ooijen, 1995. JoinMapTM version 2.0: Software for the Calculation of Genetic Linkage Maps. CPRO-DLO, Wageningen.

    Google Scholar 

  • Thomas, M.R., S. Matsumoto, P. Cain & N.S. Scott, 1993. Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theor Appl Genet 86: 173-180.

    CAS  Google Scholar 

  • Vithanage, V. & C.W. Winks, 1992. Isozymes as genetics for Macadamia. Scientia Horticult 49: 103-115.

    Article  CAS  Google Scholar 

  • Vithanage, V., K.L Anderson, C.P. Peace & M.R. Thomas, 2002. Sequence Tagged Microsatellite Site and Random Amplified Polymorphic DNA marker polymorphism in macadamia. Theor Appl Genet (submitted).

  • Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van den Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M. Zabeau, 1995. AFLP: a new technology for DNA fingerprinting. Nucleic Acids Res 23: 4407-4414.

    PubMed  CAS  Google Scholar 

  • Waldron, J., C.P. Peace, I.R. Searle, A. Furtado, N. Wade, M.W. Graham & B.J. Carroll, 2002. Randomly amplified DNA fingerprinting (RAF): a culmination of DNA marker protocols based on arbitrarily primed PCR. J Biomed Biotech 2: 141-150.

    Article  CAS  Google Scholar 

  • Weeden, N.F., 1996. Genetic studies in pea and apple: the yin and yang of mapping in plants. Hortscience 31: 1111-1116.

    CAS  Google Scholar 

  • Weeden, N.F., G.M. Timmerman, M. Hemmat, B.E. Kneen & M.A Lodhi, 1992. Inheritance and reliability of RAPD markers. In: D. Hoisington & A. McNab (Eds.), A Proceedings of the Symposium on Application of RAPD Technology to Plant Breeding, pp. 12-17. Crop Science Society of America, Minneapolis.

    Google Scholar 

  • Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531-6535.

    PubMed  CAS  Google Scholar 

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Author notes

  1. C.P. Peace

    Present address: Department of Pomology, UC Davis, Kearney Agricultural Center, 9240 S. Riverbend Ave., Parlier, CA, 93648, U.S.A

Authors and Affiliations

  1. CSIRO Plant Industry, 120 Meiers Rd, Indooroopilly, Qld, 4068, Australia

    C.P. Peace & V. Vithanage

  2. Department of Botany, University of Qld, St Lucia, Qld, 4067, Australia

    C.P. Peace & C.G.N. Turnbull

  3. School of Molecular and Microbial Sciences and School of Land and Food Sciences, University of Qld St Lucia, Qld, 4067, Australia

    C.P. Peace & B.J. Carroll

Authors
  1. C.P. Peace
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  2. V. Vithanage
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  3. C.G.N. Turnbull
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  4. B.J. Carroll
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Peace, C., Vithanage, V., Turnbull, C. et al. A genetic map of macadamia based on randomly amplified DNA fingerprinting (RAF) markers. Euphytica 134, 17–26 (2003). https://doi.org/10.1023/A:1026190529568

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  • DOI: https://doi.org/10.1023/A:1026190529568

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