Abstract
We have analysed and mapped physically the satellite I, III (subunits pvu and sau) and IV DNA sequences in cattle using in-situ hybridization. Four breeds were analysed including individuals with a chromosome number of 2n=60 and individuals with the widespread t(1;29) in the homozygous (2n=58) and heterozygous state (2n=59). All three satellite DNA families were present at the centromeres of the many but not all of the autosomal acrocentric chromosomes, and essentially absent from the sex chromosomes. In the translocated t(1;29) chromosome, the satellite DNA families showed a different pattern from that simply derived by fusion of the acrocentric autosomes and loss of satellite sequences, with no variation between breeds. A model of centromeric evolution is presented involving two independent events. Knowledge of mechanisms of translocation formation within cattle is important for a functional understanding of centromere and satellites, investigation of chromosomal abnormalities, and for understanding chromosomal fusion during evolution of other bovids and genome evolution in general.
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References
Bouvet A, Cribiu EP (1990) Analysis of synaptonemal complexes behaviour in a bull carrying the 1;29 and 9;23 Robertsonian translocations. Reprod Domestic Animals 25: 215–219.
Burkin DJ, O'Brien PCM, Broad TE et al. (1997) Isolation of chromosome-specific paints from high-resolution flow karyotypes of the sheep (Ovis aries). Chromosome Res 5: 102–108.
Chaves R, Heslop-Harrison JS, Guedes-Pinto H (2000) Centromeric heterochromatin in the cattle rob( 1;29) translocation: x-satellite sequences, in-situMspI digestion patterns, chromomycin staining and C-bands. Chromosome Res 8: 621–626.
Chaves R, Adega F, Santos S, Heslop-Harrison JS, Guedes-Pinto H (2002) In situ hybridization and chromosome banding in mammalian species. Cytogenet Genome Res 96: 113–116.
Choo KHA (1997) The Centromere. New York: Oxford University Press.
Cortadas J, Macaya G, Bernardi G (1977) An analysis of the bovine genome by density gradient centrifugation: fractionation in Cs2SO4/3,6-bis(acetatomercurimethyl) dioxane density gradient. Eur J Biochem 76: 13–19.
Eggen A, Oustry A, Vaiman D, Ferretti L, Fries R, Cribiu EP (1994) Bovine synteny group U7, previously assigned to G-banded chromosome 25, corresponds to R-banded chromosome 29. Hereditas 121: 295–300
Gallagher DS, Davis SK, De Donato M et al. (1999) A molecular cytogenetic analysis of the tribe Bovini (Artiodactyla: Bovidae: Bovinae) with an emphasis on sex chromosome morphology and NOR distribution. Chromosome Res 7: 481–492.
Garagna S, Marzilliano N, Zuccotti M, Searle JB, Capanna E, Redi CA (2001) Pericentromeric organization at the fusion point of mouse Robertsonian translocation chromosomes. Proc Natl Acad Sci USA 98: 171–175.
Gravholt C, Friedrich U, Caprani M, Jorgensen A (1992) Breakpoints in Robertsonian translocations are localized to satellite DNA by fluorescence in situ hybridization. Genomics 14: 924–930.
Gustavsson I (1969) Cytogenetics, distribution and phenotypic effects of a translocation in Swedish cattle. Hereditas 63: 68–169.
Gustavsson I (1974) Distribution and effects of the 1/29 Robertsonian translocation in cattle. J Dairy Sci 62: 825–855.
Iannuzzi L, Di Berardino D, Gustavsson I, Ferrara L, Di Meo GP (1987) Centromeric loss in translocations of centric fusion type in cattle and water buffalo. Hereditas 106: 73–81.
Iannuzzi L, Perucatti A, Di Meo GP, Ferrara L (1990) A comparison of G-and R-banding in cattle and river buffalo prometaphase chromosomes. Caryologia 43: 283–290.
Iannuzzi L, Rangel-Figueiredo T, DiMeo GP, Ferrara L (1992) A new Robertsonian translocation in cattle, rob(15;25). Cytogenet Cell Genet 59: 280–283.
ISCNDB 2000 (2001) International System for Chromosome Nomenclature of Domestic Bovids. Cytogenet Cell Genet 92: 283–299.
Jobse C, Buntjer JB, Haagsma N, Breukelman HJ, Beintema JJ, Lenstra JA (1995) Evolution and recombination of Bovine DNA repeats. J Mol Evol 41: 277–283.
Kurnit D, Shafit B, Maio J (1973) Multiple satellite deoxyribonucleic acids in the calf and their relation to the sex chromosomes. J Mol Biol 81: 273–284.
Lee C, Court DR, Cho C, Haslett JL, Lin C-C (1997) Higher-order organization of subrepeats and evolution of cervid satellite I DNA. J Mol Evol 44: 327–335.
Logue DN, Harvey MJA (1978) Meiosis and spermatogenesis in bull heterozygous for a presumptive 1/29 Robertsonian translocation. J Reprod Fertil 54: 159–165.
Macaya G, Cortadas J, Bernardi G (1978) An analysis of the bovine genome by density-gradient centrifugation. Preparation of the dG + dC-rich DNA components. Eur J Bioch 84: 179–188.
Modi WS, Gallagher DS, Womack JE (1996) Evolutionary histories of highly repeated DNA families among the Artiodactyla (Mammalia). J Mol Evol 42: 337–349.
Niebuhr E (1972) Dicentric and monocentric Robertsonian translocations in man. Humangenetik 16: 217–226.
Nijman IJ, Lenstra JA (2001) Mutation and recombination in cattle satellite DNA: A feedback model for the evolution of satellite DNA repeats. J Mol Evol 52: 361–371.
Polli E, Ginelli E, Bianchi P, Corneo G (1966) Renaturation of calf thymus satellite DNA. J Mol Biol 17: 305–308.
Popescu CP, Pech A (1991) Une bibliographie sur la translocation 1/29 de bovins dans le monde (1964-1990). Ann Zootech 40: 271–305.
Rangel-Figueiredo T, Iannuzzi L (1993) Frequency and distribution of rob (1;29) in three Portuguese cattle breeds. Hereditas 119: 233–237.
Schildkraut C, Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol 4: 430–443.
Schwarzacher T, Heslop-Harrison P (2000) Practical in situ Hybridization. Oxford: Bios.
Taparowsky E, Gerbi S (1982) Structure of 1.711 g/cm3</del> bovine satellite DNA: evolutionary relationship with satellite I. Nucl Acid Res 10: 5503–5515.
Wienberg J, Stanyon R, Nash WG (1997) Conservation of human vs. feline genome organisation revealed by reciprocal chromosome painting. Cytogenet Cell Genet 77: 211–217.
Wilson TD (1990) Identification of the 1/29 Robertsonian translocation chromosome in British Friesian cattle. Vet Rec 112: 429–432.
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Chaves, R., Adega, F., Heslop-Harrison, J.S. et al. Complex satellite DNA reshuffling in the polymorphic t(1;29) Robertsonian translocation and evolutionarily derived chromosomes in cattle. Chromosome Res 11, 641–648 (2003). https://doi.org/10.1023/A:1025952507959
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DOI: https://doi.org/10.1023/A:1025952507959