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Evolution of ribosomal DNA unit on the X chromosome independent of autosomal units in the liverwort Marchantia polymorpha

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Abstract

In the haploid dioecious liverwort, Marchantia polymorpha, the X chromosome, but not the Y, carries a cluster of ribosomal RNA genes (rDNAs). Here we show that sequences of 5S, 17S, 5.8S and 26S rDNAs are highly conserved (>99% identity) between the X chromosomal and autosomal rDNA repeat units, but the intergenic spacer sequences differ considerably. The most prominent difference is the presence of a 615-bp DNA fragment in the intergenic spacer, X615, which has accumulated predominantly in the rDNA cluster of the X chromosome. These observations suggest that the rDNA repeat unit on the X chromosome evolved independently of that on autosomes, incorporating sex chromosome-specific sequences.

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Author notes

  1. Mariko Fujishita

    Present address: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, 277-8562, Japan

  2. Kanji Ohyama

    Present address: Laboratory of Plant Molecular Genetics, Research Institute of Agricultural Resources, Ishikawa Agricultural College, 1-308, Suematsu, Nonoichi-machi, Ishikawa, 921-8836, Japan

Authors and Affiliations

  1. Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan

    Masaki Fujisawa, Tomohisa Nishio, Kiwako Hayashi, Kimitsune Ishizaki, Masataka Kajikawa, Katsuyuki T. Yamato, Hideya Fukuzawa & Kanji Ohyama

  2. Genetic Diversity Department, National Institute of Agrobiological Sciences, Kannondai, Tsukuba, 305-8602, Japan

    Shigeki Nakayama

  3. Bio-oriented Technology Research Advancement Institution, Tokyo, 105-0001, Japan

    Mariko Fujishita

Authors
  1. Masaki Fujisawa
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  2. Shigeki Nakayama
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  10. Kanji Ohyama
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Correspondence to Kanji Ohyama.

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Fujisawa, M., Nakayama, S., Nishio, T. et al. Evolution of ribosomal DNA unit on the X chromosome independent of autosomal units in the liverwort Marchantia polymorpha . Chromosome Res 11, 695–703 (2003). https://doi.org/10.1023/A:1025941206391

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  • DOI: https://doi.org/10.1023/A:1025941206391

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