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TDZ-induced somatic embryogenesis in non-responsive caryopses of rice using a short treatment with 2,4-D

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Abstract

Caryopses cultures of rice on an auxin medium (2,4-D, 20 μM) formed slow-growing tissues that failed to regenerate. The embryogenic tissue possibly lost its regeneration potential on the auxin medium. Reproducible regeneration, however, could be achieved by a short treatment with 20 μM 2,4-D for 3 days. Transfer of these caryopses to the medium containing TDZ or BA at 10 μM resulted in regeneration of somatic embryos and shoots in 30% of the cultures within 10–15 days. TDZ was better than BA for inducing shoot regeneration. Short treatment for 3 days at higher concentrations of auxin (40–80 μM) and subsequent transfer to TDZ or BA medium resulted in an increased frequency (up to 50%) of regeneration.

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References

  • Abe T &; Futsuhara Y (1986) Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theor. Appl. Genet. 72: 3–10

    Google Scholar 

  • Armstrong CL &; Green CE (1985) Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 164: 207–214

    Google Scholar 

  • Bhaskaran S &; Smith RH (1990) Regeneration in cereal tissue culture: a review. Crop Sci. 30: 1328–1336

    Google Scholar 

  • Botti C &; Vasil IK (1983) Plant regeneration by somatic embryo-genesis from plants of cultured mature embryos of Pennisetum americanum (L.) K. Schum. Z. Pflanzenphysiol. 111: 319–325

    Google Scholar 

  • Bovo OA &; Mroginski LA (1989) Somatic embryogenesis and plant regeneration from cultured mature and immature embryos of Paspalum notatum (Gramineae). Plant Sci. 65: 217–223

    Google Scholar 

  • Heyser JW, Dykes TA, DeMott KJ &; Nabors MW (1983) High frequency long term regeneration of rice from callus cultures. Plant Sci. Lett. 29: 175–182

    Google Scholar 

  • Inoue M &; Maeda E (1980) Effect of auxin and cytokinins on the occurrence of green regions in rice callus cultures. Jpn. J. Crop Sci. 49: 167–174

    Google Scholar 

  • Jones TJ &; Rost TR (1989) The developmental anatomy and ultrastructure of somatic embryos from rice (Oryza sativa L.) scutellum. Bot. Gaz. 150: 41–49

    Google Scholar 

  • Krans JV, Henning VT &; Torres KC (1982) Callus induction, maintenance and plantlet regeneration in creeping bentgrass. Crop Sci. 22: 1193–1197

    Google Scholar 

  • Krishnaraj S &; Vasil IK (1995) Somatic embryogenesis in herbace-ous monocots. In: Thorpe TA (ed) In vitro Embryogenesis in Plants (pp. 417–470). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Marousky FJ &; West SH (1990) Somatic embryogenesis and plant regeneration from cultivated mature caryopsis of bahia grass (Paspalum notatum Flugge). Plant Cell Tiss. Org. Cult. 20: 125–129

    Google Scholar 

  • Masuda K, Kudo-Shiratori A &; Inoue M (1989) Callus transforma-tion and plant regeneration from rice protoplasts purified by density gradient centrifugation. Plant Sci. 62: 237–246

    Google Scholar 

  • Morita M, Xing XH &; Unno H (1999) Synchronized shoot regene-ration of rice (Oryza sativa L.) calli on solid medium by adjustal., ment of intracellular 2,4-dichlorophenoxy acetic acid concentration. Plant Cell Rep. 18: 633–639

    Google Scholar 

  • Murashige T &; Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Murthy BNS, Murch SJ &; Saxena PK (1995) Thidiazuron induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons. Physiol. Plant. 94: 268–276

    Google Scholar 

  • Murthy BNS, Murch SJ &; Saxena PK (1998) Thidiazuron a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev. Biol. Plant 34: 267–275

    Google Scholar 

  • Ozawa K, Ling DH &; Komamine A (1996) High-frequency somatic embryogenesis from small suspension-cultured clusters of cells of an interspecific hybrid of Oryza. Plant Cell Tiss. Org. Cult. 46: 157–159

    Google Scholar 

  • Pareddy DR &; Petolino JF (1990) Somatic embryogenesis and plant regeneration from immature inflorescences of several elite inbreds of maize. Plant Sci. 67: 211–219

    Google Scholar 

  • Poonsapaya P, Nabors MW, Wright K &; Vajrabhaya M (1989) A comparison of methods for callus culture and plant regeneration of RD25 rice (Oryza sativa L.) in two laboratories. Plant Cell Tiss. Org. Cult. 16: 175–186

    Google Scholar 

  • RaghavaRam NV &; Nabors MW (1984) Cytokinin mediated long term, high frequency plant regeneration in rice tissue cultures. Z. Pflanzenphysiol. 113: 315–323

    Google Scholar 

  • Rueb S, Leneman M, Schilperoort &; Hensgens LAM (1994) Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell Tiss. Org. Cult. 36: 259–264

    Google Scholar 

  • Siriwardana S &; Nabors MW (1983) Tryptophan enhancement of somatic embryogenesis in rice. Plant Physiol. 73: 142–146

    Google Scholar 

  • Sticklen MB, Rumpho ME &; Kennedy RA (1989) Media con-ditioning to convert non-embryogenic rice calli to embryogenic calli. Int. Rice Res. Rep. 14: 8–9

    Google Scholar 

  • Torello WA, Symington AG &; Rufner R (1984) Callus initiation; plant regeneration and evidence of somatic embryogenesis in red fescue. Crop Sci. 24: 1037–1040

    Google Scholar 

  • Utomo HS, Croughan TP &; Croughan SS (1996) Growth and regenerability of long-term suspensions cultures of US rice cultivar Mercury. Plant Cell Tiss. Org. Cult. 44: 155–159

    Google Scholar 

  • Vikrant &; Rashid A (2002a) Somatic embryogenesis from im-mature and mature embryos of a minor millet Paspalum scrobiculatum L. Plant Cell Tiss. Org. Cult. 69: 71–77

    Google Scholar 

  • Vikrant &; Rashid A (2002b) Induction of multiple shoots by thidiazuron from caryopsis cultures of minor millet (Paspalum scrobiculatum L.) and its effect on the regeneration of em-bryogenic callus cultures. Plant Cell Rep. 21: 9–13

    Google Scholar 

  • Visser C, Qureshi JA, Gill R &; Saxena PK (1992) Morphoregulat-ory role of thidiazuron. Plant Physiol. 99: 1704–1707

    Google Scholar 

  • Ye X, Babili Salim Ali, Kloti A, Zhang J, Lucca P, Beyer P &; Potrykus I (2000) Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 187: 303–305

    Google Scholar 

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  1. Department of Botany, University of Delhi, Delhi, 110007, India

    Aparna Gairi & A. Rashid

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  1. Aparna Gairi
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  2. A. Rashid
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Correspondence to A. Rashid.

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Gairi, A., Rashid, A. TDZ-induced somatic embryogenesis in non-responsive caryopses of rice using a short treatment with 2,4-D. Plant Cell, Tissue and Organ Culture 76, 29–33 (2004). https://doi.org/10.1023/A:1025864605846

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