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Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda

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Abstract

Somatic embryogenesis is the most important in vitro culture system for conifer propagation. However, Pinus taeda has been considered recalcitrant to somatic embryogenesis in commercial scale-up. The study of biochemical and physiological aspects of cell growth could lead to a better understanding of somatic embryogenesis in this species. In the present work, we investigated the cell growth dynamics, intracellular levels of proteins, starch and polyamines in suspension cultures of Pinus taeda established in plant growth regulator-free medium (BM0) and in medium supplemented with 2 μM 2,4-dichlorophenoxyacetic acid, 0.5 μM 6-benzylaminopurine and 0.5 μM Kinetin (BM2). Cell cultures growing in BM0 medium showed an increase in the sedimented cell volume from 3.77 to 17.73 ml after 24 days of culture. Those cultured in BM2 medium showed an increase in the sedimented cell volume from 4.23 to 25.17 ml after 20 days of culture. Intracellular proteins levels increased during the exponential growth phase and starch levels decreased until the exponential phase, followed by a synthesis up to the stationary phase, in both BM0 and BM2 media. Highest putrescine levels occurred in cultures growing in BM0 medium and this was associated with the low cellular growth.

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Authors and Affiliations

  1. Plant Cell Biology Laboratory, Department of Botany, IB-University of São Paulo CP, 11461, São Paulo, Brazil

    Vanildo Silveira, Eny Iochevet Segal Floh & Walter Handro

  2. Plant Developmental Physiology and Genetic Laboratory, Department of Plant Science, Federal University of Santa Catarina, CP 476, Florianópolis, Brazil

    Miguel Pedro Guerra

Authors
  1. Vanildo Silveira
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  2. Eny Iochevet Segal Floh
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  3. Walter Handro
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  4. Miguel Pedro Guerra
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Correspondence to Miguel Pedro Guerra.

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Silveira, V., Iochevet Segal Floh, E., Handro, W. et al. Effect of plant growth regulators on the cellular growth and levels of intracellular protein, starch and polyamines in embryogenic suspension cultures of Pinus taeda . Plant Cell, Tissue and Organ Culture 76, 53–60 (2004). https://doi.org/10.1023/A:1025847515435

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