Abstract
A comparison of similarity indices between 64 durum wheat cultivars calculated using pedigree analysis and RAPD method showed a correspondence between these two approaches to estimation of genetic diversity. The associations between the results of RAPD clustering and coefficients of parentage (χ2 test) and the coefficient of correlation between similarity matrices were statistically significant. However, the correlation was rather weak while pedigree analysis and RAPD method did not yield completely identical estimates of genetic diversity in the set of cultivars studied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Thormann, C.E., Ferreira, M.E., Camargo, L.E.A., et al., Comparison of RFLP and RAPD Markers to Estimating Genetic Relationships within and among Cruciferous Species, Theor. Appl. Genet., 1994, vol. 88, pp. 973-980.
Powell, W., Morgante, M., Andre, C., et al., The Comparison of RFLP, RAPD, AFLP, and SSR (Microsatellite) Markers for Germplasm Analysis, Mol. Breed., 1996, vol. 2, pp. 225-238.
Martynov, S.P. and Dobrotvorskaya, T.V., A Study of Genetic Diversity in Wheat Using the Genetic Resources Information and Analysis System, GRIS, Genetika (Moscow), 2000, vol. 36, no. 2, pp. 138-144.
Souza, E. and Sorrells, M.E., Pedigree Analysis of North American Oat Cultivars Released from 1951 to 1985, Crop Sci., 1989, vol. 29, pp. 595-601.
Metakovsky, E.V. and Novoselskaya, A.Yu., Gliadin Allele Identification in Common Wheat: I. Methodological Aspects of the Analysis of Gliadin Patterns by One-Dimensional Polyacrylamide Gel Electrophoresis, J. Genet. Breed., 1991, vol. 45, pp. 317-324.
Tai, T.H. and Tanksley, S.D., A Rapid and Inexpensive Method for Isolation of Total DNA from Dehydrated Plant Tissue, Plant. Mol. Biol. Repr., 1990, vol. 8, pp. 297-303.
Nei, M. and Li, W.H., Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases, Proc. Natl. Acad. Sci. USA, 1979, vol. 76, pp. 5269-5273.
Rohlf, F.J., NTSYSpc: Numerical Taxonomy and Multivariate Analysis System, Version 2.02c, New York: Exeter Software, 1998.
Manjarrez-Sandoval, P., Carter, T.E., Webb, D.M., and Burton, J.W., RFLP Genetic Similarity Estimates and Coefficient of Parentage as Genetic Variance Predictors for Soybean Yield, Crop Sci., 1997, vol. 37, no. 3, pp. 698-703.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kudryavtsev, A.M., Martynov, S.P., Broggio, M. et al. Relevance of RAPD Analysis for Revealing Phylogenetic Relationships between Cultivars of Durum Wheat Triticum durum Desf.. Russian Journal of Genetics 39, 1043–1052 (2003). https://doi.org/10.1023/A:1025779202500
Issue Date:
DOI: https://doi.org/10.1023/A:1025779202500