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Reaction of substituted phenols with thermostable laccase bound to Bacillus subtilis spores

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Abstract

A strain of B. subtilis produced 1.8 times more laccase on sporulation medium than on non-sporulation medium. Spores oxidized mono- and di-methoxyphenols (0.1 mM) at 50 °C. The half-life of laccase bound to spores was about 2 d and the substrate was repeatedly removed by spores recovered from the reaction mixture.

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Engineering, Miyazaki University, Miyazaki, 889-2192, Japan

    Jun Hirose, Masako Nasu & Haruhiko Yokoi

Authors
  1. Jun Hirose
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  2. Masako Nasu
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  3. Haruhiko Yokoi
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Correspondence to Jun Hirose.

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Hirose, J., Nasu, M. & Yokoi, H. Reaction of substituted phenols with thermostable laccase bound to Bacillus subtilis spores. Biotechnology Letters 25, 1609–1612 (2003). https://doi.org/10.1023/A:1025663931019

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  • DOI: https://doi.org/10.1023/A:1025663931019

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