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Protein sorting and expression of a unique soybean cotyledon protein, GmSBP, destined for the protein storage vacuole

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Abstract

The initial biochemical characterization of the soybean sucrose-binding protein, GmSBP, within our lab and others produced several incongruous characteristics that required a re-characterization of GmSBP via sequence homology, cell biology, immunolocalization, and semi-quantitative analysis. The GmSBP proteins share amino acid sequence homology as well as putative structural homology with globulin-like seed storage proteins. A comparison to the major soybean seed storage proteins, glycinin and β-conglycinin established several storage protein-like characteristics for GmSBP. All three proteins were present in a prevacuolar compartment and protein storage vacuole. All three proteins increased in expression during seed development and are remobilized during germination. Quantitatively, the relative concentrations of GmSBP, β-conglycinin (α/α′ subunits), and glycinin (acidic subunits) indicated that GmSBP contributes 19-fold less to the stored nitrogen. The quantitative differences between GmSBP and glycinin may be attributed to the unconserved order and spacing of cis-acting regulatory elements present within the promoter regions. Ultimately, GmSBP is transported to the mature protein storage vacuole. The biological function of GmSBP within the protein storage vacuole remains uncertain, but its localization is a remnant of its evolutionary link to a globulin-like or vicilin-like ancestor that gave rise to the 7S family of storage proteins.

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Authors and Affiliations

  1. Program in Plant Physiology, School of Molecular Sciences, Washington State University, Pullman, WA, 99164-4234, USA

    Aaron Elmer

  2. Biosciences Research Laboratory USDA-ARS, Fargo, ND, 58105-5674, USA

    Wun Chao

  3. School of Molecular Biosciences, Washington State University, Pullman, WA, 99164-4234, USA

    Howard Grimes

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  1. Aaron Elmer
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  2. Wun Chao
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  3. Howard Grimes
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Correspondence to Howard Grimes.

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Elmer, A., Chao, W. & Grimes, H. Protein sorting and expression of a unique soybean cotyledon protein, GmSBP, destined for the protein storage vacuole. Plant Mol Biol 52, 1089–1106 (2003). https://doi.org/10.1023/A:1025483809791

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