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Transformation of Atriplex gmelini plants from callus lines using Agrobacterium tumefaciens

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Abstract

Atriplex gmelini plants were regenerated via organogensis from hypocotyl explants. Callus lines were induced from the hypocotyl explants on Linsmaier and Skoog (LS) medium supplemented with 1 μM benzyladenine and 5 μM α-naphthaleneacetic acid in the dark. Shoots were regenerated from the callus lines on LS medium supplemented with 20 μM thidiazuron and 0.1 μM α-naphthaleneacetic acid under a high-intensity light condition (450 μmol m−2 s−1). The regenerated shoots were rooted on LS medium without growth regulators to obtain fully developed plants. We succeeded in transforming Atriplex gmelini from callus lines using Agrobacterium tumefaciens.

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Authors and Affiliations

  1. Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa Nagoya, 464-8601, Japan

    Akio Uchida, Kenji Nagamiya & Tetsuko Takabe

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  1. Akio Uchida
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  2. Kenji Nagamiya
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  3. Tetsuko Takabe
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Correspondence to Tetsuko Takabe.

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Uchida, A., Nagamiya, K. & Takabe, T. Transformation of Atriplex gmelini plants from callus lines using Agrobacterium tumefaciens . Plant Cell, Tissue and Organ Culture 75, 151–157 (2003). https://doi.org/10.1023/A:1025086505120

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  • DOI: https://doi.org/10.1023/A:1025086505120

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