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Temporal progression of gene expression responses to salt shock in maize roots

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Abstract

Using a cDNA microarray containing 7943 ESTs, the behavior of the maize root transcriptome has been monitored in a time course for 72 h after imposition of salinity stress (150 mM NaCl). Under these conditions, root sodium amounts increased faster than in leaves, and root potassium decreased significantly. Although the overall free amino acid concentration was not affected, amino acid composition was changed with proline and asparagine increasing. Microarray analysis identified 916 ESTs representing genes whose steady-state RNA levels were significantly altered at various time points, corresponding to 11% of the ESTs printed. The response of the transcriptome to sub-lethal salt stress was rapid and transient, leading to a burst of changes at the three-hour time point. The salt-regulated ESTs represented 472 tentatively unique genes (TUGs), which, based on functional category analysis, are involved in a broad range of cellular and biochemical activities, prominent amongst which were transport and signal transduction pathways. Clustering of regulated transcripts based on the timing and duration of changes suggests a structured succession of induction and repression for salt responsive genes in multiple signal and response cascades. Within this framework, 16 signaling molecules, including six protein kinases, two protein phosphatases and eight transcription factors, were regulated with distinct expression patterns by high salinity.

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Authors and Affiliations

  1. Department of Plant Sciences, University of Arizona, Tucson, AZ, 85721, USA

    Hong Wang & David W. Galbraith

  2. Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ, 85721, USA

    Saori Miyazaki, Kiyoshi Kawai & Hans J. Bohnert

  3. Department of Plant Biology and Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL, 61801, USA

    Saori Miyazaki & Hans J. Bohnert

  4. Department of Plant Sciences, Molecular Biophysics, Canada

    Michael Deyholos

  5. Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2E9, Canada

    Michael Deyholos

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  1. Hong Wang
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  2. Saori Miyazaki
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Correspondence to Hans J. Bohnert.

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Wang, H., Miyazaki, S., Kawai, K. et al. Temporal progression of gene expression responses to salt shock in maize roots. Plant Mol Biol 52, 873–891 (2003). https://doi.org/10.1023/A:1025029026375

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