Skip to main content
Log in

Comparative Study of Microbial Communities from Cultured and Natural Populations of the Mussel Mytilus trossulus in Peter the Great Bay

  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

The 525 strains of heterotrophic bacteria isolated from natural and cultured populations of the mussel Mytilus trossulus and the surrounding seawater were identified to a genus level on the basis of phenotypic analysis and the fatty acid composition of cell lipids. Gram-negative isolates were dominated by six genera of the family Enterobacteriaceae and by the genera Pseudoalteromonas, Vibrio, Photobacterium, Cytophaga/Flavobacterium/Bacteroides, Pseudomonas, and Moraxella. Gram-positive isolates were mainly represented by the genus Streptomyces. The taxonomic compositions of natural and cultured populations of the mussel M. trossulus in Peter the Great Bay were similar.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  1. Dupont, J., Jehl-Pietri, C., and Mnard, D., Comparative Study of Bacterial and Viral Faecal Contamination in Shellfish: Demonstration of Seasonal Variations, Biomed. Lett., 1992, vol. 47, pp. 329-335.

    Google Scholar 

  2. Chan, S.S. and Ho, K.W., Bacterial Contamination of the Pacific Oysters (Crassostrea gigas) Cultured in Deep Bay, Hong Kong, Environ. Technol., 1993, vol. 14, pp. 861-867.

    Google Scholar 

  3. Colwell, R.R. and Liston, J., Microbiology of Shellfish: Bacteriological Study of the Natural Flora of Pacific Oysters (Crassostrea gigas), Appl. Microbiol., 1960, vol. 8, pp. 104-109.

    Google Scholar 

  4. Prieur, D., Mvel, G., and Nicolas, J.-L., Plusguellea, A., and Vigneulle, M., Interactions between Bivalve Molluscs and Bacteria in the Marine Environment, Oceanogr. Mar. Biol. Annu. Rev., 1990, vol. 28, pp. 277-352.

    Google Scholar 

  5. Sugita, H., Tanaomi, H., Kobashi, T., and Deguchi, I., The Bacterial Flora of the Coastal Bivalves Nippon suisan gakkaishi, Bull. Jap. Soc. Sci. Fish, 1981, vol. 47,no. 5, pp. 655-661.

    Google Scholar 

  6. Ortigosa, M., Garay, E., and Pujalte, M.J., Numerical Taxonomy of Aerobic, Gram-Negative Bacteria Associated to Oysters and Surrounding Seawater in Mediterranean Coast, Syst. Appl. Microbiol., 1995, vol. 17, pp. 588-600.

    Google Scholar 

  7. Youschimizu, M. and Kimura, T., Study on Intestinal Microflora of Salmonids, Fish Pathol., 1976, vol. 10,no. 2, pp. 243-259.

    Google Scholar 

  8. The Prokaryotes: A Handbook of the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, Starr, M.P. et al., Eds., Berlin: Springer, 1992.

    Google Scholar 

  9. Ivanova, E.P., Romanenko, L.A., and Mikhailov, V.V., Morskie bakterii semeistva Alteromonadaceae (Marine Bacteria of the Family Alteromonadaceae), Vladivostok: Dal'nauka, 2001.

    Google Scholar 

  10. Marmur, J., A Procedure for the Isolation of Deoxyribonucleic Acid from Microorganisms, J. Mol. Biol., 1961, no. 3, pp. 208-218.

  11. Owen, R.J., Hill, R.L., and Lapage, S.P., Determination of DNA Base Composition from Melting Profiles in Dilute Buffers, Biopolymers, 1969, vol. 7, pp. 503-516.

    Google Scholar 

  12. Carreau, J.P. and Dubacq, J.P., Adaptation of a Macro-Scale Method to the Micro-Scale for Fatty Acid Methyl Trans-Esterification of Biological Lipid Extracts, J. Chromatogr., 1978, vol. 151, pp. 384-390.

    Google Scholar 

  13. Pujalte, M.J., Ortigosa, M., Macian, M.C., and Garay, E., Aerobic and Facultative Anaerobic Heterotrophic Bacteria Associated to Mediterranean Oysters and Seawater, Int. Microbiol., 1999, vol. 2, pp. 259-266.

    Google Scholar 

  14. Lechevalier, H. and Lechevalier, M.P., Chemotaxonomic Use of Lipids: An Overview, Microbial Lipids, Ratledge, C. and Wilkinson, S.C., Eds., London: Academic, 1988, vol. 1, pp. 869-902.

    Google Scholar 

  15. Ivanova, E.P., Zhukova, N.V., Svetashev, V.I., Gorshkova, N.M., et al., Evaluation of Phospholipid and Fatty Acid Compositions as Chemotaxonomic Markers of Alteromonas-like Proteobacteria, Curr. Microbiol. 2000, vol. 41, pp. 341-345.

    Google Scholar 

  16. Urdaci, M.C., Marchand, M., and Grimont, P.A., Characterization of 22 Vibrio Species by Gas Chromatography Analysis of Their Cellular Fatty Acids, Res. Microbiol., 1990, vol. 141, pp. 437-452.

    Google Scholar 

  17. Oyauzu, H. and Komagata, K., Grouping of Pseudomonas Species on the Basis of Cellular Fatty Acids Composition and the Quinone System with Special Reference to the Existence of 3-Hydroxy Fatty Acids, J. Gen. Appl. Microbiol., 1983, vol. 29, pp. 17-40.

    Google Scholar 

  18. Dzierzewicz, Z., Cwalina, B., Kurkiewicz, S., Chodurek, E., and Wilczok, T., Intraspecies Variability of Cellular Fatty Acids among Soil and Intestinal Strains of Desulfovibrio desulfuricans, Appl. Environ. Microbiol. 1996, vol. 62,no. 9, pp. 3360-3365.

    Google Scholar 

  19. Ivanova, E.P. and Mikhailov, V.V., Microorganisms Associated with the mussel Crenomytilus grayanus and Their Hydrolytic Activity, Mikrobiologiya, 1992, vol. 61,no. 3, pp. 514-519.

    Google Scholar 

  20. Kutzner, H.J., The Family Streptomycetaceae, The Prokaryotes, Starr, M. et al., Eds., Berlin: Springer, 1981, vol. 2, pp. 2028-2090.

    Google Scholar 

  21. Mikhailov, V.V., Kuznetsova, T.A., and Elyakov, G.B., Morskie mikroorganizmy i ikh vtorichnye biologicheski aktivnye metabolity (Marine Microorganisms and Their Biologically Active Metabolites), Vladivostok: Dal'nauka, 1999.

    Google Scholar 

  22. Ugolev, A.M., Evolyutsiya pishchevareniya i printsipy evolyutsii funktsii. Elementy sovremennogo funktsionalizma (The Evolution of Digestion and the Principles of the Evolution of Functions: Elements of Modern Functionalism), Leningrad: Nauka, 1985.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beleneva, I.A., Zhukova, N.V. & Maslennikova, E.F. Comparative Study of Microbial Communities from Cultured and Natural Populations of the Mussel Mytilus trossulus in Peter the Great Bay. Microbiology 72, 472–477 (2003). https://doi.org/10.1023/A:1025005025620

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025005025620

Navigation