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Metabolic and phylogenetic profiles of microbial communities from a mariculture base on the Chinese Guangdong coast

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Abstract

This study examined the phylogenetic and metabolic features of microbial communities in Baisha Bay mariculture areas of Nan’ao Island, a mariculture base on the Chinese Guangdong coast. Large seaweed (Gracilaria lemaneiformis) cultivation zones (LZ), fish culture zones (FZ), and control zones (CZ) were sampled. According to community-level physiological profiling, d-mannitol, d-mannitol/α-d-lactose, and d-mannitol/α-cyclodextrin were the carbon sources with the highest utilization rates in CZ, LZ, and FZ, respectively (R s  > 1.9). Denaturing gradient gel electrophoresis analysis showed that the dominant bacteria in CZ and LZ were Alphaproteobacteria (39.9 and 34.5%, respectively), whereas in FZ they were Flavobacteria (32.2%). The Shannon diversity in CZ and LZ was significantly higher than in FZ (P < 0.05). Some opportunistic pathogens, such as Vibrio, Pseudoalteromonas and Loktanella, were abundant in FZ. The microbial communities and most of the dominant bacteria in FZ were more affected by environmental factors including salinity, chemical oxygen demand and total phosphorus. Some bacteria, such as Brevundimonas and Polaribater, have the potential to degrade agar in seaweed, and were isolated from samples rich in algae which are dominant in LZ. The cultivation of G. lemaneiformis created the notable structures and functions of macroalgae-associated bacterial communities that were less affected by the environmental factors examined.

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Acknowledgements

This research was supported by the Joint Project of the Nature Science Foundation of China-Guangdong (U1301235), the Chinese Special Fund for Agro-scientific Research in the Public Interest (201403008), the Fund of the Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, the Ministry of Agriculture, P. R. China (FREU2015-08), and the Natural Science Foundation of Hainan Province (20163148).

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Authors and Affiliations

  1. Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology and Environment, Guangdong Province, South China Sea Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, People’s Republic of China

    Xiaojuan Hu, Guoliang Wen, Yucheng Cao & Zhuojia Li

  2. Institute of Hydrobiology, Jinan University, Guangzhou, 510632, People’s Republic of China

    Xiaojuan Hu & Yufeng Yang

  3. Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, 572018, People’s Republic of China

    Xiaojuan Hu

  4. Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People’s Republic of China

    Yingxue Gong

  5. Institute of Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma, Norman, OK, 73019, USA

    Zhili He

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  1. Xiaojuan Hu
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  2. Guoliang Wen
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Hu, X., Wen, G., Cao, Y. et al. Metabolic and phylogenetic profiles of microbial communities from a mariculture base on the Chinese Guangdong coast. Fish Sci 83, 465–477 (2017). https://doi.org/10.1007/s12562-017-1073-5

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