Abstract
Seedlings were grown in vitro from fertilized eggs and immature embryos of the Siberian stone pine. Cultivation of megagametophytes on a hormone-containing Murashige-Skoog medium from the egg formation until the globular embryo stage made it possible to manipulate fertilization and embryogenesis. Immature embryos are the most promising for in vitrocultivation. Their maturation and germination proceed within seven days of cultivation. When zygotic embryos were cultivated, adventitious buds were formed from cells at the cotyledon base and tips. When adventitious buds were subcultivated on a medium containing benzylaminopurine and naphthylacetic acid, organogenic callus and shoots were formed. Thus, cultivation of megagametophytes and embryos of the Siberian stone pine led to the completion of embryogenesis and formation of viable of seedlings.
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Aderkas, P. and Bonga, J.M., Morphological Definition of Phenocritical Period for Initiation of Haploid Embryogenic Tissue From Explants of Larix decidua, Somatic Cell Genetics of Woody Plants, Ahuja, M.R., Ed., Dordrecht: Kluwer Academic, 1988, pp. 29-38.
Aderkas, P., Bonga, J.M., Klimaszewska, K., and Owens, J., Comparison of Larch Embryogeny in vivo and in vitro, Woody Plants Biotechnology, Placerville, CA: Plenum, 1991, pp. 139-155 (Ser. A: Life Sciences, vol. 210).
Arnold, S. and Hakman, I., Effect of Sucrose on Initiation of Embryogenic Callus Cultures from Mature Zygotic Embryos of Picea abies (L.) Karst. (Norway Spruce), J. Plant Physiol., 1985, vol. 122, no. 3, pp. 261-265.
Arnold, S. and Hakman, I., Regulation of Somatic Embryo Development in Picea abies by Abscisic Acid (ABA), J.?Plant Physiol., 1988, vol. 132, no. 2, pp. 164-169.
Becwar, M.R., Nagmani, R., and Wann, S.R., Initiation of Embryogenic Cultures and Somatic Embryo Development in Loblolly Pine (Pinus taeda), Can. J. Forest Res., 1990, vol. 20, no. 6, pp. 810-817.
Behrendt, U. and Zoglauer, K., Boron Controls Suspensor Development in Embryogenic Cultures of Larix deciduas, Physiol. Plant., 1996, vol. 97, no. 3, pp. 321-326.
Calixto, F. and Pais, S.M., Adventitious Shoot Formation and Plant Regeneration from Pinus pinaster Sol. Ex Aiton, In vitro Cell. Dev. Biol., 1997, vol. 33, no. 2, pp. 119-124.
Cell and Tissue Culture in Forestry, Bonga, J.M. and Durzan, D.J., Eds., Dordrecht.: Martinus Nijhoff, 1987.
Drake, P.M.W. and John, A., Cytokinin Pulse-Mediated Shoot Organogenesis from Cotyledons of Sitra spruse [Picea sitchensis (Bong.) Carr.] and High Frequency in vitro Rooting of Shoots, Plant Cell Tiss. Org. Cult., 1997, vol. 50, no.?2, pp. 147-151.
Egertsdotter, U. and Arnold, S., Development of Somatic Embryos in Norway Spruce, J. Experim. Bot., 1998, vol. 49, no. 319, pp. 155-162.
Fernando, D.D. and Owens, J.N., Development of Resistance to White Pine Blister Rust through in vitro Fertilization, Internat. Conference “Breeding and Genetic Resources of Five-Needle Pines,” Oregon, USA: JUFRO, Working Party, 2001, p. 112.
Fernando, D.D., Owens, J.N., and Aderkas, P., in vitro Fertilization from Co-Cultured Pollen Tubes and Female Gametophytes of Douglas Fir (Pseudotsuge menziesii), Theor. Appl. Genet., 1998, vol. 10, no. 4, pp. 1057-1063.
Finer, J.J., Kriebel, H.B., and Becwar, M.R., Initiation of Embryogenic Callus and Suspension Cultures of Eastern White Pine (Pinus strobes L.), Plant Cell Rep., 1989, vol. 8, no. 2, pp. 203-206.
Guevin, T.G. and Kirby, E.G., Induction of Embryogenesis in Cultured Mature Zygotic Embryos of Abies fraseri (Pursh) Poir, Plant Cell Tis. Org. Cult., 1997, vol. 49, no. 3, pp. 219-222.
Gupta, R.K. and Dursan, D.J., Somatic Polyembryogenesis from Callus of Mature Sugar Pine Embryos, Biotech., 1986, vol. 4, no. 5, pp. 643-645.
Hakman, I. and Arnold, S., Somatic Embryogenesis and Plant Regeneration from Suspension Cultures of Picea glauca (White Spruce), Physiol. Plant., 1988, vol. 72, no. 3, pp. 579-587.
Hakman, I. and Fowke, L.C., Somatic Embryogenesis in Picea glauca (White Spruce) and P. mariana (Black Spruce), Can. J. Bot., 1987, vol. 65, no. 4, pp. 656-659.
Hakman, I., Fowke, L.C., Arnold, S., and Eriksson, T., The Development of Somatic Embryos in Callus Cultures Initiated from Immature Embryos of Picea abies, Plant Sci., 1985, vol. 38, no. 1, pp. 53-59.
Havel, L. and Dursan, D.J., Apoptosis during Diploid Parthenogenesis and Early Somatic Embryogenesis of Norway Spruce, Internat. J. Plant Sci., 1996, vol. 157, no. 1, pp. 8-16.
Hong, L., Boulay, M., Gupta, P.K., and Dursan, D.J., Variations in Somatic Polyembryogenesis: Induction of Adventitious Embryonal-Suspensor Masses on Developing Douglas Fir Embryos, Woody Plants Biotechnology, Plaserville, CA: Plenum, 1991, pp. 105-121 (Ser. A: Life Sciences, vol. 210).
Iroshnikov, A.I., Variations of some morphological features and ecological-physiolological characteristics in the Siberian Stone Pine, Izmenchivost' drevesnykh rastenii Sibiri (Variability of Tree Plants of Siberia), Krasnoyarsk: Inst. Lesa i Drevesiny, Sib. Otd. Akad. Nauk SSSR, 1974, pp. 77-103.
Ivanov, V.M., Geterotsiklicheskie Azotsoderzhashchie Azosoedineniya (Heterocyclic Nitrogen-Containing Azocompounds), Moscow: Nauka, 1982.
Jourdain, I., Lelu, M.A., and Label, P., Hormonal Changes during Growth of Somatic Embryogenic Masses in Hybrid Larch, Plant Physiol. Biochem. (Paris), 1997, vol. 35, no. 9, pp. 741-749.
Kashin, A.S., Blyudneva, E.A., Sedletskaya, O.V., and Grigor'eva, V.V., Specific Features of Development of Megagametes in Apomictic and Sexual Forms of the Asteraceae in the Culture of Unfertilized Gynaecia in vitro, Fiziol. Rast. (Moscow), vol. 47, no. 4, pp. 548-554.
Klimaszewska, K. and Smith, D.R., Maturation of Somatic Embryos of Pinus strobes Is Promoted by a Nigh Concentration of Gellan Gum, Physiol. Plant., 1997, vol. 100, no. 4, pp. 949-957.
Kuznetsova, G.V., Specific Features of Growth and Development of Stone Pines on Sylvicultures of Middle Siberia, Cand. Sci. (Biol.) Dissertation, Krasnoyarsk: In-t lesa SO RAN, 2001.
Li, X.Y., Huang, F.H., and Gbur, E.E., Effect of Basal Medium, Growth Regulators and Phytagel Concentration on Initiation of Embryogenic Cultures from Immature Zygotic Embryos of Loblolly Pine (Pinus taeda L.), Plant Cell Reports, 1997, vol. 17, no. 4, pp. 298-301.
Liao, Y.K. and Amerson, H.V., Slash Pine (Pinus elliottii Engelm.) Somatic Embryogenesis. 1. Initiation of Embryogenic Cultures from Immature Zygotic Embryos, New For., 1995, vol. 10, no. 2, pp. 145-163.
Lu, C.Y. and Thorpe, T.A., Somatic Embryogenesis and Plantlet Regeneration in Cultured Immature Embryos of Picea glauca, Plant Physiol., 1987, vol. 128, no. 4, pp. 297-302.
Minina, E.G. and Larionova, N.A., Morfogenez i proyavlenie pola u khvoinykh (Morphogenesis and Sex Expression in Coniferales), Moscow: Nauka, 1979.
Murashige, T. and Skoog, F., A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiol. Plant, 1962, vol. 15, no. 4, pp. 473-497.
Nagmani, R. and Bonga, J.M., Embryogenesis in Subcultured Callus of Larix deciduas, Can. J. Forest Res., 1985, vol. 15, no. 5, pp. 1088-1091.
Nagmani, R., Johnson, M.A., and Dinus, J., Effect of Explant and Media on Initiation, Maintenance, and Maturation of Somatic Embryos in Pseudotsuga menziesii (Mirb.) Franco (Douglas-Fir), Woody Plant Biotechnology, Plaserville, CA: Plenum, 1991, pp. 171-178 (Ser. A: Life Sciences, vol. 210).
Ogita, S., Kubo, T., and Fushitani, M., Anatomical Characteristics in Early Embryogenesis from Immature Embryos of Larix leptolepis, For. Res. Environment (Tokyo), 1997, no. 35, pp. 45-51.
Pausheva, Z.P., Praktikum po tsitologii rastenii (A Manual of Plant Cytology), Moscow: Kolos, 1980.
Roberts, D.R., Webster, F.B., Flinn, B.S., et al., Application of Somatic Embryogenesis to Clonal Propagation of Interior Spruce, Woody Plant Biotechnology, 1991, pp. 157-169 (Ser. A: Life Sciences, vol. 210).
Salaj, J. and Salajova, T., Histological Observations of Adventitious Bud Development in Pinus nigra, Biologia, 1998, vol. 53, no. 1, pp. 107-110.
Salopen, B., Milakovic, T.T., Mihaljevic, S., and Jelaska, S., Storage Product Accumulation during the Maturation of Picea omorika (Pan.) Purk. Somatic Embryos, Perodic. Biol., 1997, vol. 99, no. 1, pp. 117-124.
Tang, W., Ouyano, F., and Guo, Z.C., Plant Regeneration through Organogenesis From Callus Induced from Mature Zygotic Embryos of Loblolly Pine, Plant Cell Reports, 1998, vol. 17, no. 6/7, pp. 557-560.
Tret'yakova, I.N., Embriologiya khvoinykh: fiziologicheskie aspekty (Embryology of Coniferales: Physiological Aspects), Novosibirsk: Nauka, 1990.
Tret'yakova, I.N., Nevzorov, V.N., Golubev, I.V., and Filippova, I.P., Biological Characteristics of the Siberian Stone Pine of Varying Sizes, Materialy Mezhdunar. nauch. prakt. konf. “Genetika i selektsiya—na sluzhbe lesu” (Materials of the International Conference “Genetics and Selection to the Service of Forest”), Voronezh: Rodnaya rech', 1997, pp. 349-353.
Vagner, M., Vondrakova, Z., Strnadova, Z., and Eder, J., Mones during Early Stages of Somatic Embryogenesis of Picea abies, Adv. Horticult. Sci., 1998, vol. 12, no. 1, pp. 11-18.
Woody Plant Biotechnology, Ahuja, M.R., Ed., Placerville, CA: Plenum, 1991 (Ser. A. Life Sciences, vol. 210).
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Tret'yakova, I.N., Novoselova, N.V. Specific Features of Development of Megagametophytes and Embryos of the Siberian Stone Pine in vitro . Russian Journal of Developmental Biology 34, 232–240 (2003). https://doi.org/10.1023/A:1024996516398
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DOI: https://doi.org/10.1023/A:1024996516398