Abstract
Collagen induced the synthesis of collagenolytic enzymes in the culture of Aspergillus flavus. Enzyme activity of the culture increased during storage and passage on a medium with this inductor. We developed a method for isolation and purification of collagenolytic enzymes and obtained two electrophoretically homogenous enzyme preparations belonging to neutral thermolabile collagenolytic metalloproteinases.
Similar content being viewed by others
REFERENCES
N. S. Demina and S. V. Lysenko, Mikrobiologiya, 65,No. 3, 293-304 (1996).
Ya. E. Dunaevskii, E. B. Pavlyukova, T. N. Gruban', et al., Biokhimiya, 61,No. 10, 1904-1909 (1996).
O. A. Klimova and V. Yu. Chebotarev, Byull. Eksp. Biol. Med., 128,No. 10, 391-396 (1999).
N. S. Landau and N. S. Egorov, Mikrobiologiya, 65,No. 1, 42-47 (1996).
M. B. Yakovleva and V. L. Kozel'tsev, Prikl. Biokhim. Mikrobiol., 30,No. 1, 121-126 (1994).
Y. P. Lee and T. Takahashi, Anal. Biochem., 14, 71-77 (1996).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sukhosyrova, E.A., Nikitina, Z.K., Yakovleva, M.B. et al. Characteristics of Collagenolytic Enzymes Secreted by Deuteromycete Fungi Aspergillus Flavus . Bulletin of Experimental Biology and Medicine 135, 447–451 (2003). https://doi.org/10.1023/A:1024911308278
Issue Date:
DOI: https://doi.org/10.1023/A:1024911308278