Abstract
Regeneration in oil palm was achieved through somatic embryogenesis/organogenesis from embryo-derived callus. Callus was induced from mature embryos of the cross 281 (D)×18 (P) on modified MS medium supplemented with 2,4-D (113.12 μM) and 2-iP (14.76 μM). The embryogenic calluses obtained were transferred to Blaydes medium supplemented with 2,4-D (0.045 μM) and one of the following growth regulators: TDZ (4.54 μM), zeatin riboside (2.85 μM), putrescine (1 mM) and spermine (100 μM). Secondary somatic embryogenesis was found to occur in media supplemented with polyamines. The efficiency of formation of somatic embryos, secondary somatic embryos and shoot meristemoids were significantly higher in putrescine containing medium. Histological studies were also undertaken.
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Rajesh, M., Radha, E., Karun, A. et al. Plant regeneration from embryo-derived callus of oil palm – the effect of exogenous polyamines. Plant Cell, Tissue and Organ Culture 75, 41–47 (2003). https://doi.org/10.1023/A:1024679910085
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DOI: https://doi.org/10.1023/A:1024679910085