Skip to main content
SpringerLink
Log in

The effect of osmotic potential on anther culture in spring wheat (Triticum aestivum)

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

This study was conducted to determine the effect of osmotic potential in a modified 85D12 medium on both callus induction and plant regeneration in the anther culture of two wheat genotypes, cv. Chris and cv. Pavon. Altering the medium osmotic potential by changing the carbohydrate source and concentration or by adding a non-metabolized osmoticum appeared to have the greatest potential for improving anther-derived green plant production. The medium osmotic potentials were varied (-0.67 to −2.30 MPa) by altering sucrose and PEG concentration. Both osmotica affected callus production, with −0.9 to −1.4 MPa media producing the most calluses. Callus production depended on genotype and osmoticum. Only PEG concentration affected green plant regeneration. The greatest number of green plants (11.5 plants per 100 anthers in cv. Chris) was obtained with 0.0125 M of PEG. This experiment suggested that a low level of PEG in the medium was beneficial for producing green plants from wheat anthers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abd El-Maksoud MM, Karsai I & Bedö Z (1993) Agronomic traits of wheat lines developed by the doubled haploid, single seed descent and pedigree methods after three cycles of selection. Acta Agr. Hung. 42: 377–382

    Google Scholar 

  • Brown DCW, Leung DWM & Thorpe TA (1979) Osmotic requirement for shoot formation in tobacco callus. Physiol. Plant. 46: 36–41

    Google Scholar 

  • Brown DCW & Thorpe TA (1980) Changes in water potential and its components during shoot formation in tobacco callus. Physiol. Plant. 49: 83–87

    Google Scholar 

  • Davidson DJ & Chevalier PM (1987) Influence of polyethylene glycol-induced water deficits on tiller production in spring wheat. Crop Sci. 27: 1185–1187

    Google Scholar 

  • Dodds JH & Roberts LW (1985) Experiments in Plant Tissue Culture. In: Experiments in Plant Tissue Culture (pp. 156–171). Cambridge Universtiy Press, New York

    Google Scholar 

  • Dube SD (1984) Genotypic differences for callus formation in immature anthers of wheat Triticum aestivum. Crop Improv. 11: 64–65

    Google Scholar 

  • Hoekstra S, van Bergen S, van Brouwershaven IR, Schilperoort RA & Heidekamp F (1996) The interaction of 2,4-D application and mannitol pre-treatment in anther and microspore culture of Hordeum vulgare L. cv. Igri. J. Plant Physiol. 148: 696–700

    Google Scholar 

  • Hu H, Xi Z, Ouyang J, Hao S, He M, Xu Z & Zou M (1980) Chromosome variation of pollen mother cell of pollen-derived plants in wheat (Triticum aestivum L.). Sci. Sin. 23: 905–912

    Google Scholar 

  • Ilíc-Grubor K, Attree SM & Fowke LC (1998) Induction of microspore-derived embryos of Brassica napus L. with poly-ethylene glycol (PEG) as osmoticum in a low sucrose medium. Plant Cell Rep. 17: 329–333

    Google Scholar 

  • Kao KN (1993) Viability, cell division and microcallus formation of barley microspores in culture. Plant Cell Rep. 12: 366–369

    Google Scholar 

  • Karsai I & Bedö Z (1998) Relationship between anther culture response and aluminium tolerance in wheat (Triticum aestivum L.). Euphytica 100: 249–252

    Google Scholar 

  • Kovács G & Barnabás (1997) Selection for frost tolerance in anther culture-derived embryos and regeneration of frost-tolerant fertile DH plants in winter wheat. Acta Agr. Hung. 45: 285–293

    Google Scholar 

  • Kudirka DT, Schaeffer GW & Baenziger PS (1989) Stability of ploidy in meristems of plants regenerated from anther calli of wheat (Triticum aestivum L. em Thell.). Genome 32: 1068–1073

    Google Scholar 

  • Lawlor DW (1970) Absorption of polyethylene glycol by plants and their effects on plant growth. New Phytol. 69: 501–513

    Google Scholar 

  • Lazar MD, Chen THH, Scoles GJ & Kartha KK (1987) Immature embryo and anther culture of chromosome addition lines of rye in Chinese spring wheat. Plant Sci. 51: 77–81

    Google Scholar 

  • Levitt J (1969) Introduction to Plant Physiology. In: Introduction to Plant Physiology (pp. 44–50). Mosby Company, Saint Louis

    Google Scholar 

  • Liang GH, Xu A & Tang H (1987) Direct generation of wheat haploids via anther culture. Crop Sci. 27: 336–339

    Google Scholar 

  • Linossier L, Veisseire P, Cailloux F & Coudret A (1997) Effects of abscisic acid and high concentrations of PEG on Hevea brasiliensis somatic embryo development. Plant Sci. 124: 183–191

    Google Scholar 

  • Luckett DJ & Darvey NL (1992) Utilization of microspore culture in wheat and barley improvement. Aust. J. Bot. 40: 807–828

    Google Scholar 

  • McGregor LM & McHughen A (1990) The influence of various cultural factors on anther culture of four cultivars of spring wheat (Triticum aestivum L.). Can. J. Plant Sci. 70: 183–191

    Google Scholar 

  • Metz SG, Sharma HC, Armstrong TA & Mascia PN (1988) Chromosome doubling and aneuploidy in anther-derived plants from two winter wheat lines. Genome 30: 177–181

    Google Scholar 

  • Palmer RG & Heer H (1973) A root-tip squash technique for soybean chromosomes. Crop Sci. 13: 389–391

    Google Scholar 

  • Rains DW(1989) Plant tissue and protoplast culture: application to stress physiology and biochemistry. In: Jones HG, Flowers TJ & Jones MB (eds) Plants Under Stress (pp. 181–196). Cambridge University Press, London

    Google Scholar 

  • Schaeffer GW, Baenziger PS & Worley J (1979) Haploid plant development from anthers and in vitro embryo culture of wheat. Crop Sci. 19: 697–702

    Google Scholar 

  • Shivanna KR, Saxena NP & Seetharama N (1997) An improvised medium for in vitro pollen germination and pollen tube growth of chickpea. Int. Chickpea and Pigeonpea Newsletter 4: 28–29

    Google Scholar 

  • Thörn EC (1988) Effect of melibiose and polyethylene glycol on anther culture response of barley. In: Int. Congress Genetic Manipulation and Plant Breeding-Biotechnol. Breed. (p. 8)

  • Tschaplinski TJ, Gebre GM, Dahl JE, Roberts GT & Tuskan GA (1995) Growth and solute adjustment of calli of Populus clones cultured on nutrient medium containing polyethylene glycol. Can. J. For. Res. 25: 1425–1433

    Google Scholar 

  • van der Weele CM, Spollen WG, Sharp RE & Baskin TI (2000) Growth of Arabidopsis thaliana seedlings under water deficit studied by control of water potential in nutrient-agar media. J. Exp. Bot. 51: 1555–1562

    Google Scholar 

  • Zhang HQ & Croes AF (1982) A new medium for pollen germination in vitro. Acta Botanica Neerlandica 31: 113–119

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Basic Sciences, Chonbuk National University, Chonju, 561- 756, South Korea

    Tae-Jin Kang

  2. Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University, Honju, 3 561- 756, South Korea

    Moon-Sik Yang

  3. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

    Edward L. Deckard

Authors
  1. Tae-Jin Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Moon-Sik Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edward L. Deckard
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tae-Jin Kang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kang, TJ., Yang, MS. & Deckard, E.L. The effect of osmotic potential on anther culture in spring wheat (Triticum aestivum). Plant Cell, Tissue and Organ Culture 75, 35–40 (2003). https://doi.org/10.1023/A:1024643526923

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1024643526923

Search

Navigation