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Subcellular Distribution of Homer 1b/c in Relation to Endoplasmic Reticulum and Plasma Membrane Proteins in Purkinje Neurons

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Abstract

The subcellular distribution of endoplasmic reticulum proteins (IP3R1 and RYR), plasma membrane(PM) proteins (mGluR1 and PMCA Ca2+-pump), and scaffolding proteins, such as Homer 1b/c, was assessed by laser scanning confocal microscopy of rat cerebellum parasagittal sections. There appeared to be two classes of Ca2+ stores, nonjunctional Ca2+ stores and junctional Ca2+ stores, possibly referable to central cisternae/tubules and sub-PM cisternae, respectively, in soma, dendrites, and dendritic spines. Only some IP3R1s appeared to be part of multimeric, junctional Ca2+ signaling networks, whose composition is shown to include PMCA, mGluR1, Homer 1b/c and, not always, RYR1.

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Authors and Affiliations

  1. Dipartimento di Scienze Biomediche Sperimentali dell', Università degli Studi di Padova, viale G. Colombo 3, 35121, Padova, Italy

    Dorianna Sandonà, Alessandra Scolari & Pompeo Volpe

  2. Department of Molecular Neurobiology, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokane dai, Minato-ku, Tokyo, 108-8639, Japan

    Katsuiko Mikoshiba

Authors
  1. Dorianna Sandonà
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  2. Alessandra Scolari
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  3. Katsuiko Mikoshiba
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  4. Pompeo Volpe
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Correspondence to Pompeo Volpe.

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Sandonà, D., Scolari, A., Mikoshiba, K. et al. Subcellular Distribution of Homer 1b/c in Relation to Endoplasmic Reticulum and Plasma Membrane Proteins in Purkinje Neurons. Neurochem Res 28, 1151–1158 (2003). https://doi.org/10.1023/A:1024264025401

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