Abstract
The gene expression plasmid, pET-Lmluc, for the fusion protein of the hyaluronan binding domain from human TSG-6 [product of tumor necrosis factor (TNF)-stimulated gene-6] and luciferase from Renilla reniformis was constructed. The fused gene was expressed in Escherichia coli and the resulted insoluble Lm-luc fusion protein was purified and refolded to recover both the hyaluronan binding capability and the luciferase activity. Hyaluronan as low as 1 ng ml−1 was detected by using the indirect enzymatic immunological assay with the refolded Lm-luc fusion protein.
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Chang, TS., Wan, HM., Chen, CC. et al. Fusion protein of the hyaluronan binding domain from human TSG-6 with luciferase for assay of hyaluronan. Biotechnology Letters 25, 1037–1040 (2003). https://doi.org/10.1023/A:1024178110310
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DOI: https://doi.org/10.1023/A:1024178110310