Abstract
Culture conditions are described for high frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis Max. Petiole explants formed embryogenic calluses at a frequency of 53% when cultured on B5 medium supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Leaf explants formed embryogenic calluses at a frequency of 21% when cultured at a combination of 4.52 μM 2,4-D and 2.22 μM 6-benzyladenine. Cell suspension cultures were established with petiole-derived embryogenic calluses using liquid B5 medium with 4.52 μM 2,4-D. Upon plating onto B5 basal medium, cell suspension cultures produced numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.
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References
Castillo B & Smith MAL (1997) Direct somatic embryogenesis from Begonia gracilis explants. Plant Cell Rep. 16: 385-388
Colombo ML & Bosisio E (1996) Pharmacological activities of Chelidonium majus L. (Papaveraceae). Pharmacol. Res. 33: 127-134
Colombo ML & Tome F (1991) Production of sanguinarine by Chelidonium majus callus culture. Planta Med. 57: 428-429
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158
Kelkar SM & Krishnamurthy KV (1998) Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link. Plant Cell Rep. 17: 721-725
Kim SW, Min BW & Liu JR (1999) High frequency plant regeneation from immature ovule-derived embryogenic cell suspension cultures of Chelidonium majus var. asiaticum. Plant Cell Tiss. Org. Cult. 56: 125-129
Marchant R, Davey MR, Lucas JA & Power JB (1996) Somatic embryogenesis and plant regeneration in floribunda rose (Rosa hybrida L.) cvs. Trumpeter and Glad Tidings. Plant Sci. 120: 95-105
Panzer A, Hamel E, Joubert AM, Bianchi PC & Seegers JC (2000) Ukrain TM, a semisynthetic Chelidonium majus alkaloid derivative, acts by inhibition of tubulin polymerization in normal and malignant cell lines. Cancer Lett. 160: 149-157
Rogelj B, Popovic T, Ritonja A, Strukelj B & Brzin J (1998) Chelidocystatin, a novel phytocystatin from Chelidonium majus. Phytochemistry 49: 1645-1649
Tome F & Colombo ML (1992) Characterization of a suspension culture of Chelidonium majus L. on growth and accumulation of sanguinarine. Biochem. Physiol. Pflanz. 188: 116-120
Wakhlu AK & Sharma RK (1998) Somatic embryogenesis and plant regeneration in Heracleum candicansWall. Plant Cell Rep. 17: 866-869
Wildi E, Schaffner W & Berger Buter K (1998) In vitro propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and from inflorescence buds. Plant Cell Rep. 18: 336-340
Woo JW, Huh GH, Ahn MY, Kim SW & Liu JR (1996) Somatic embryogenesis and plant regeneration in pedicel explant cultures of Chelidonium majus var. asiaticum. Kor. J. Plant Tiss. Cult. 23: 363-366
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Kim, S., In, D., Kim, T. et al. High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis . Plant Cell, Tissue and Organ Culture 74, 163–167 (2003). https://doi.org/10.1023/A:1023997627578
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DOI: https://doi.org/10.1023/A:1023997627578