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A novel potato defence-related alcohol:NADP+ oxidoreductase induced in response to Erwinia carotovora

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Abstract

Identification of Solanum tuberosum genes responsive to culture filtrates (CF) from Erwinia carotovora subsp. carotovora led to the isolation of a full-length cDNA with high sequence similarity to several alcohol dehydrogenases. Accumulation of transcripts corresponding to this defence-related alcohol dehydrogenase (drd-1) was rapidly induced in CF-treated and wounded plants. The gene was also responsive to molecules involved in defence signalling such as salicylic acid, methyl jasmonate and ethylene. To elucidate the biochemical function of DRD-1, its cDNA was expressed in Escherichia coli. Enzymatic assays revealed that DRD-1 is an alcohol:NADP+ oxidoreductase with preference for various aromatic and aliphatic aldehydes. The enzyme exhibited high activity with several aldehydes including 2-methoxybenzaldehyde, 3-methoxybenzaldehyde, salicylaldehyde, o-vanillin, cinnamaldehyde, hydrocinnamaldehyde, hexanal and octanal. Identification of the reaction product by thin-layer chromatography confirmed the reduction of aldehydes to alcohols. Enzymatic activity measured with 2-methoxy-benzaldehyde as a substrate was increased in salicylic acid- or methyl jasmonate-treated plants. These data suggest that DRD-1 may play an important role in potato defence response to Erwinia carotovora.

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Authors and Affiliations

  1. Department of Biosciences, Division of Genetics, University of Helsinki, Box 56, 00014, Finland

    Marcos Montesano, Heidi Hyytiäinen & E. Tapio Palva

  2. Departamento de Biología Molecular-Unidad Asociada a Facultad de Ciencias, Universidad de la República, Instituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, CP. 11600, Montevideo, Uruguay

    Marcos Montesano & Rodolfo Wettstein

  3. Institute of Biotechnology, University of Helsinki, Box 56, 00014, Finland

    E. Tapio Palva

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  1. Marcos Montesano
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  2. Heidi Hyytiäinen
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Montesano, M., Hyytiäinen, H., Wettstein, R. et al. A novel potato defence-related alcohol:NADP+ oxidoreductase induced in response to Erwinia carotovora . Plant Mol Biol 52, 177–189 (2003). https://doi.org/10.1023/A:1023981631596

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