Abstract
A study was made on the use of a mixed microalgal consortium to degrade p-nitrophenol. The consortium was obtained from a microbial community in a waste container fed with the remains and by-products of medium culture containing substituted aromatic pollutants (nitrophenols, chlorophenols, fluorobenzene). After selective enrichment with p-nitrophenol (p-NP), followed by an antibiotic treatment, an axenic microalgal consortium was recovered, which was able to degrade p-nitrophenol. At a concentration of 50 mg L−1, total degradation occurred within 5 days. Two species, Chlorella vulgaris var. vulgaris f. minuscula and Coenochloris pyrenoidosa, were isolated from the microalgal consortium. The species were able to accomplish p-NP biodegradation when cultured separately, although Coenochloris pyrenoidosa was more efficient, achieving the same degradation rate as the original axenic microalgal consortium. When Coenochloris pyrenoidosa was associated with Chlorella vulgaris in a 3:1 ratio, complete removal of the nitro-aromatic compound occurred within three days. This is apparently the first report on the degradation of a nitro-aromatic compound by microalgae.
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References
Alexander M. 1994. Biodegradation and Bioremediation. Academic Press, San Diego, CA, USA.
Bull A.T. and Slater J.H. 1982. Historical perspectives on mixed cultures and microbial communities. In: Bull A.T. and Slater J.H. (eds), Microbial Interactions and Communities. Vol. 1. Academic Press, London, pp. 1-14.
Caldeira M., Heald S.C., Carvalho M.F., Vasconcelos I., Bull A.T. and Castro P.M.L. 1999. 4-Chlorophenol degradation by a bacterial consortium: development of a granular activated carbon biofilm reactor. Appl. Microbiol. Biotechnol. 52: 722-729.
Cerniglia C.E., Gibson D.T. and Van Baalen C. 1980. Oxidation of naphthalene by cyanobacteria and microalgae. J. gen. Microbiol. 116: 495-500.
Craigier J.S., McLachlan J. and Towers H.N. 1965. A note on the fission of aromatic ring by algae. Can. J. Bot. 43: 1589-1590.
Hardman D.J. 1991. Biotransformation of halogenated compounds. Critical Reviews in Biotechnology 11: 1-40.
Jingi L. and Houtian O. 1992. Degradation of azo dyes by algae. Envir. Pollut. 75: 273-278.
Lovell C.R., Eriksen N.T., Lewitus A.J. and Chen Y.P. 2002. Resistance of the marine diatom Thalassiosira sp. to toxicity of phenolics compounds. Mar. Ecol. Progr. Ser. 229: 11-18.
Luther M. 1990. Degradation of different substituted aromatic compounds as nutrient sources by the green alga Scenedesmus obliquus. Dechema Biotechnol. Conferences 4: 613-615.
Semple K.T. and Cain R.B. 1995. Metabolism of phenols by Ochromonas danica. FEMS Microb. Letts. 133: 253-257.
Semple K.T. and Cain R.B. 1996. Biodegradation of phenols by the alga Ochromonas danica. Appl. envir. Microbiol. 62: 1265- 1273.
Semple K.T., Cain R.B. and Schmidt S. 1999. Biodegradation of aromatic compounds by microalgae. FEMS Microb. Letts. 170 291-300.
Shashirekha S., Uma L. and Subramanian G. 1997. Phenol degradation of the marine cyanobacterium Phormidium valderianum BDU 30501. J. ind. Microbiol. Biotech. 19: 130-133.
Spain J.C. 1995. Biodegradation of nitroaromatic compounds. Annu. Rev. Microbiol. 49: 523-555.
Tikoo V., Scragg A.H. and Shales S.W. 1997. Degradation of pentachlorophenol by microalgae. J. chem. Technol. Biotechnology 68: 425-431.
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Lima, S.A., Castro, P.M. & Morais, R. Biodegradation of p-nitrophenol by microalgae. Journal of Applied Phycology 15, 137–142 (2003). https://doi.org/10.1023/A:1023877420364
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DOI: https://doi.org/10.1023/A:1023877420364