Abstract
The plasmid profile of two thermophilic bacterial strains isolated from recreation thermal springs in Jordan has been investigated. These strains are Streptococcus thermophilus and Bacillus sp1, which have been isolated from Zerka – Maeen and Himma hot springs respectively. Supercoiled and circular plasmid forms were detected, explaining the effect of DNA conformation on the mobility of the plasmid in the agarose gel electrophoresis. Two plasmids have been isolated and characterized by restriction endonucleases to facilitate their use as cloning vectors in thermophilic strains. The sizes of the plasmids were approximately 3 kb (from Streptococcus thermophilus) and 7 kb (from Bacillus spl). These plasmids were then digested with three different restriction enzymes (EcoRI, Bam HI, and HindIII), one of which was found to possess a single site for both plasmids, this enzyme is EcoRI.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akimkina T., Ivanow, P., Kostrows, S., Sokolova, T., Bonch-Osmolovskaya, L., Firman, K., Dutta, C.F. & Mc Clellen, J. 1999 A Highly conserved plasmid from the extreme thermophile Thermotoga maritima MC24 is a member of a family of plasmids distributed wordwide, Plasmid 40, 236–240.
Becker, D.A., Glass, K.A. & Starzyk, M.J. 1986 Isolation and curing of extrachromosomal DNA in Thermus aquaticus. Microbios 48, 97–99.
Birnboim, H.C. & Doly, J.A. 1979 rapid alkaline extraction procedure for screening plasmid DNA. Nucleic Acids Research 7, 1513–1523.
Charbonnier, F., Erauso, G., Prieur, D. & Forterre, P. 1995 Purification of plasmids from thermophilic and hyperthermophilic Archaea In: A laboratory Manual. eds. Robb, F.T. & Place, A.R. pp. 87–90. Woodbury, New York; Cold Spring Harbor Laboratory Press. ISBN 0-87969-440-8.
Cork, D. & Khalil, A. 1995 Detection, isolation and stability of megaplasmid encoded chloroaromatic herbicide degrading genes within psuedomonas species. Advances in Applied Microbiology 40, 289–321.
Eberhard, M.D., Vasquez, C., Valenzuela, P., Vicuna, R. & Yudelevich, A. 1981 Physical characterization of a plasmid (pTTI) isolated from Thermus thermophilus. Plasmid 6, 1–6.
Hishinuma, F., Tanaka, T. & Sakaguchi, K. 1978 Isolation of extrachromosomal deoxyribonucleic acids from extremely thermophilic bacteria. Journal of General Microbiology 104, 193–199.
Holmes, D.S. & Quigley, M. 1981 A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114, 193–197.
Khalil, A.B., Salim, M. & Sallal, A.K. 1998 Enumeration of thermotolerant bacteria from recreational thermal ponds in Jordan. International Journal of Cytogenetics and Biological Research 96, 57–63.
Koh, C.-K. 1985 Detection and purification of plasmids present in Thermus strains from Iceland hot springs. MIRCEN Journal 1, 77–81.
Munster, M.J., Munster, A.P. & Sharp, R.J. 1985 Incidence of plasmids in Thermus sp. Isolated in Yellowstone National Park. Applied and Environmental Microbiology 50, 1325–1327.
Raven, N.D.H. & Williams, R.A.D. 1985 Isolation and partial characterization of two cryptic plasmids from an extreme thermophile. Biochemical Society Transactions 13, 214–218.
Vasquez, C., Venegas, A. & Vicuna, R. 1981 Characterization and cloning of a plasmid isolated from the extreme thermophile Thermus flavus AT-62. Biochemistry International 3, 291–229.
Witholt, B., Van Heerikhuizen, H. & De Leij, L. 1976 How does lysozyme penetrate through the bacterial outer membrane? Biochemica et Biophysica Acta 443, 534–544.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Khalil, A.B., Anfoka, G.H. & Bdour, S. Isolation of plasmids present in thermophilic strains from hot springs in Jordan. World Journal of Microbiology and Biotechnology 19, 239–241 (2003). https://doi.org/10.1023/A:1023692531885
Issue Date:
DOI: https://doi.org/10.1023/A:1023692531885