Abstract
Histochemical methods were used to detect differently directed changes in the metabolic activity of neurons in the anterior hypothalamic nuclei in rats during hyperthermia, fever, and hypothermia. Hyperthermia induced by high temperatures was associated with increases in the activities of enzymes involved in energy metabolism, with increases in RNA contents in neurons in the supraoptic, paraventricular, and median preoptic nuclei of the anterior hypothalamus of the rat, which is evidence for increases in metabolic activity in the neurons of these nuclei. Endotoxin-induced fever was accompanied by decreases in the metabolic activity of neurons in the median preoptic nucleus, while activity in neurons of the supraoptic and paraventricular nuclei showed no significant change. The development of hypothermia induced by low temperatures was characterized by decreases in the metabolic activity of neurons in the supraoptic, paraventricular, and median preoptic nuclei of the anterior hypothalamus. It is suggested that the differently directed changes in metabolic activity in the neurons of the anterior hypothalamus in hyperthermia, fever, and hypothermia are associated with their roles in the central mechanisms of thermoregulation (median preoptic nucleus) and neurosecretory processes (supraoptic and paraventricular nuclei).
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REFERENCES
G. A. Akimov, N. V. Alishev, V. A. Bernshtein, and V. A. Bukov, Whole Body Cooling [in Russian], Meditsina, Moscow (1977).
A. N. Buldakova, “Neurosecretory activity of the hypothalamus of the guinea pig in total hypothermia,” in: Hypothalamic Regulation of Autonomic Functions [in Russian], Vishcha Shkola, Kiev (1970).
S. V. Vladimirov and G. A. Polyakova, “The hypothalamo-hypophyseal neurosecretory system of dogs in conditions of solar thermal overheating,” Arkh. Anat. Gistol. Émbriol., 57, No. 10, 74–77 (1969).
V. N. Gurin, Mechanisms of Fever [in Russian], Nauka i Tekhnika, Minsk (1993).
A. V. Epifantsev, “The effects of low temperatures on the functional state of the brain,” in: Mechanisms of Some Pathological Processes [in Russian], Vishcha Shkola, Rostov-on-Don (1974).
V. N. Kazakov, E. V. Gaidarova, I. É. Kuznetsov, and L. V. Natrus, “Morphofunctional changes in neurons in the preoptic region and mediobasal hypothalamus in hyperthermia,” Arkh. Klin. Éksperim. Med., 8, No. 2, 143–148 (1999).
N. K. Kormilitsyna, “The role of the paraventricular nuclei of the hypothalamus in responses of the hypophyseal-adrenocortical systems in cold adaptation,” Fiziol. Zh. SSSR, 74, No. 7, 933–937 (1988).
Yu. F. Pastukhov and Z. G. Nevretdinova, “Changes in thermoregulation, sleep, and blood thyroid hormone concentrations in the ‘critical’ period of entry into hibernation in mammals,” Zh. Évolyuts. Biokhim. Fiziol., 27, No. 2, 211–217 (1991).
N. I. Tarapata, E. V. Gaidarova, B. G. Popov, and S. V. Zyablitsev, “Morphofunctional changes in the neuroendocrine system in hyperthermia,” Arkh. Klin. Éksperim. Med., 99, No. 1, 50–53 (2000).
J. A. Boilant and J. D. Hardy, “The effect of spinal and skin temperatures on the firing rate and local thermosensitivity of preoptic neurons,” J. Physiol. (London), 240, No. 3, 639–660 (1974).
J. A. Boulant, “Hypothalamic neurons regulating body temperature,” in: APS Handbook of Physiology, M. J. Fregly and C. M. Blatteis (Eds.), Oxford University Press, New York (1996).
J. Bligh, “Neural models of temperature regulation,” in: Temperature Regulation in Mammals and Other Vertebrates, North-Holland, Amsterdam (1973).
T. Hori, M. Shibata, and T. Nakashima, “Effect of interleukin-1 and arachidonate on the preoptic and anterior hypothalamic neurons,” Brain Res. Bull., 20. No. 1, 75–82 (1988).
T. Horn, M. F. Wilkinson, R. Landgraf, and O. J. Pittman, “Reduced febrile responses to pyrogens after lesions of the hypothalamic paraventricular nucleus,” Amer. J. Physiol., 267, No. 1, Part 2, R323–R328 (1994).
K. P. Ivanov, L. P. Dymnikova, and E. Khalilov, “The convergence onto posterior hypothalamic neurons of signals from thermoreceptors,” J. Therm. Biol., 6, No. 1, 37–41 (1981).
K. Kanosue, K. Niwa, P. D. Andrew, H. Yasuda, M. Yanase, H. Tanaka, and K. Matsumara, “Lateral distribution of hypothalamic signals controlling thermoregulatory vasomotor activity and shivering in rats,” Amer. J. Physiol., 260, No. 3, Part 2, R486–R493 (1991).
T. S. Kilduff, J. D. Miller, C. M. Radeke, F. R. Sharp, and H. C. Heller, “14C-2-deoxyglucose uptake in the ground squirrel brain during entrance to and arousal from hibernation,” J. Neurosci., 10, No. 7, 2463–2475 (1990).
R. M. Lechan, R. Toni, B. D. Clark, J. G. Cannon, A. R. Shaw, C. A. Dinarello, and S. Reichlin, “Immunoreactive interleukin-1-beta localization in the rat forebrain,” Brain Res., 514, No. 1, 135–140 (1990).
A. Morimoto and N. Murakami, “[14C]deoxyglucose incorporation into rat brain regions during hypothalamic or peripheral thermal stimulation,” Amer. J. Physiol., 248, No. 1, Part 2, R84–R92 (1985).
A. Morimoto, T. Ono, T. Watanabe, and N. Murakami, “Activation of brain regions of rats during fever,” Brain Res., 381, No. 1, 100–105 (1986).
A. Morimoto, T. Nakamori, T. Ono, T. Watanabe, Y. Sakai, and N. Murakami, “Changes in [14C]deoxyglucose incorporation into rat brain regions during heat-seeking behavior in the cold environment,” Physiol. Behav., 38, No. 2, 275–282 (1986).
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, New York (1982).
E. Pearse, Histochemistry, Churchill Ltd, London (1960).
C. B. Saper and C. D. Breder, “The neurologic basis of fever,” New Eng. J. Med., 330, No. 26, 1880–1886 (1994).
R. Sapolsky, C. Rivier, G. Yamamoto, P. Plotsky, and W. Vale, “Interleukin-1 stimulates the secretion of hypothalamic corticotropinreleasing factor,” Science, 238, No. 4826, 522–524 (1987).
R. Shymusiak and E. Satinoff, “Acute thermoregulatory effects of unilateral electrolytic lesions of the medial and lateral preoptic area in rats,” Physiol. Behav., 28, No. 1, 70–161 (1982).
M. Shibata, “Hypothalamic neuronal responses to cytokines,” Yale J. Biol. Med., 63, No. 2, 147–156 (1990).
L. Sokoloff, “Local cerebral energy metabolism: its relationship to local functional activity and blood flow,” Bull. Schweiz. Acad. Med. Wiss., 36, No. 1–3, 71–91 (1980).
V. Y. Vasilenko, “Thermosensitivity. An intrinsic property of hypothalamic neurons,” J. Therm. Biol., 19, No. 3, 219–236 (1994).
M. T. Wong-Riley, M. M. Merzenich, and P. A. Leake, “Changes in endogenous enzymatic reactivity to DAB induced by neuronal inactivity,” Brain Res., 141, No. 1, 185–192 (1978).
P. Yarowsky, M. Kadekaro, and L. Sokoloff, “Frequency-dependent activation of glucose utilization in the superior cervical ganglion by electrical stimulation of cervical sympathetic trunk,” Proc. Natl. Acad. Sci. USA, 80, No. 13, 4179–4183 (1983).
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Ekimova, I.V. Changes in the Metabolic Activity of Neurons in the Anterior Hypothalamic Nuclei in Rats during Hyperthermia, Fever, and Hypothermia. Neurosci Behav Physiol 33, 455–460 (2003). https://doi.org/10.1023/A:1023459100213
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DOI: https://doi.org/10.1023/A:1023459100213